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Mapping the speciation of iron in Pseudomonas aeruginosa biofilms using scanning transmission X-ray microscopy

Title data

Hunter, Ryan C. ; Hitchcock, Adam P. ; Dynes, James J. ; Obst, Martin ; Beveridge, Terry J.:
Mapping the speciation of iron in Pseudomonas aeruginosa biofilms using scanning transmission X-ray microscopy.
In: Environmental Science & Technology. Vol. 42 (2008) Issue 23 . - pp. 8766-8772.
ISSN 0013-936X
DOI: https://doi.org/10.1021/es801642z

Abstract in another language

An important feature of microbial communities is the spatial heterogeneity of extracellular chemistry. Scanning transmission X-ray microscopy (STXM) was used to map the spatial distribution of iron species throughout Pseudomonas aeruginosa biofilms to assess the influence of chemical heterogeneity on biomineralization. P. aeruginosa biofilms were treated with Fe(III)-amended media. Speciation and quantitative mapping using STXM image sequences in the Fe 2p3/2 (L3) absorption edge region revealed both Fe(II) and Fe(III) in localized microenvironments. Fe(III) was mainly associated with cell surfaces, while small amounts of Fe(II) was found in the extracellular space. Biofilms were also characterized using C 1s edge STXM image sequences. Anaerobic growth assays and confocal microscopy revealed the inability of P. aeruginosa to directly reduce Fe(III), implicating indirect iron reduction mechanisms in the formation of fine-grained, multivalent minerals. These studies suggest that geochemical microenvironments found throughout microbial communities are even more complex than previously believed.

Further data

Item Type: Article in a journal
Refereed: Yes
Additional notes: BAYCEER135589
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Earth Sciences > Heisenberg Professorship - Experimental Biogeochemistry
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Earth Sciences > Heisenberg Professorship - Experimental Biogeochemistry > Heisenberg Professorship - Experimental Biogeochemistry - Univ.-Prof. Dr. Martin Obst
Research Institutions
Research Institutions > Research Centres
Research Institutions > Research Centres > Bayreuth Center of Ecology and Environmental Research- BayCEER
Faculties
Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Earth Sciences
Result of work at the UBT: No
DDC Subjects: 500 Science
500 Science > 550 Earth sciences, geology
Date Deposited: 13 Aug 2020 13:14
Last Modified: 15 Sep 2020 08:53
URI: https://eref.uni-bayreuth.de/id/eprint/56495