Titlebar

Export bibliographic data
Literature by the same author
plus on the publication server
plus at Google Scholar

 

TEM-specimen preparation of cell/mineral interfaces by Focused Ion Beam milling

Title data

Obst, Martin ; Gasser, Philippe ; Mavrocordatos, Denis ; Dittrich, Maria:
TEM-specimen preparation of cell/mineral interfaces by Focused Ion Beam milling.
In: American Mineralogist. Vol. 90 (2005) Issue 8-9 . - pp. 1270-1277.
ISSN 1945-3027
DOI: https://doi.org/10.2138/am.2005.1743

Abstract in another language

Picocyanobacteria were found to play an important role in calcite precipitation in oligotrophic lakes. In this study, investigations on the interface between cyanobacteria and attached biogenic calcite crystals have been performed to gain further insights into the mechanisms of nucleation of these precipitates. Ultramicrotomy, the conventional preparation technique of thin sections for Transmission Electron Microscopy (TEM) investigations, often fails when working on heterogeneous samples containing soft organic material and hard minerals. Thus, in this study the thin sections were prepared using Focused Ion Beam (FIB) milling. This approach is usually applied in material sciences but until recently was not very common in environmental research. Different analytical TEM methods like Electron Spectroscopic Imaging (ESI) and Electron Energy Loss Spectrometry (EELS) were used to test the suitability of FIB-milling for the preparation of organic/inorganic interface specimens. With this approach we were able to analyze both organic and the inorganic phases of the same sample. Elemental maps of the samples were also calculated. By analyzing the structure of the C K-absorption edge, the different bonding forms of the organic carbon cell and the inorganic carbon of the crystal could be clearly distinguished.

Further data

Item Type: Article in a journal
Refereed: Yes
Additional notes: BAYCEER135618
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Earth Sciences > Heisenberg Professorship - Experimental Biogeochemistry
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Earth Sciences > Heisenberg Professorship - Experimental Biogeochemistry > Heisenberg Professorship - Experimental Biogeochemistry - Univ.-Prof. Dr. Martin Obst
Research Institutions
Research Institutions > Research Centres
Research Institutions > Research Centres > Bayreuth Center of Ecology and Environmental Research- BayCEER
Faculties
Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Earth Sciences
Result of work at the UBT: No
DDC Subjects: 500 Science
500 Science > 550 Earth sciences, geology
Date Deposited: 14 Aug 2020 06:14
Last Modified: 15 Sep 2020 08:53
URI: https://eref.uni-bayreuth.de/id/eprint/56501