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Direct Monitoring of organic vapours with amperometric enzyme gas sensors

Title data

Hämmerle, Martin ; Hilgert, Karin ; Achmann, Sabine ; Moos, Ralf:
Direct Monitoring of organic vapours with amperometric enzyme gas sensors.
In: Biosensors and Bioelectronics. Vol. 25 (2010) Issue 6 . - pp. 1521-1525.
ISSN 1873-4235
DOI: https://doi.org/10.1016/j.bios.2009.10.022

Official URL: Volltext

Project information

Project title:
Project's official titleProject's id
No informationHA 4424/1-3

Project financing: Deutsche Forschungsgemeinschaft

Abstract in another language

In this study, amperometric enzyme gas sensors for direct monitoring of organic vapours (formaldehyde, ethanol and phenol) are presented using exemplarily different sensing strategies: NADH detection, H2O2 detection and direct substrate recycling, respectively. The presented sensor configurations allow the selective, continuous, online monitoring of organic vapours without prior accumulation or sampling of the analyte. The gaseous samples are provided as headspace above aqueous solutions. The concentration in the gas phase was calculated from the concentration in solution at room temperature according to the respective Henry constants given in the literature. The enzymes employed are NAD-dependent formaldehyde dehydrogenase [EC 1.2.1.46] from Pseudomonas putida, alcohol oxidase [EC 1.1.3.13] from Pichia pastoris, and tyrosinase [EC 1.14.18.1] from mushroom. The gas diffusion working electrodes used in the sensors are based on a porous, hydrophobic PTFE membrane (exposed geometric electrode area: 1.77 cm2) covered with a porous layer of gold, platinum or graphite/Teflon. Detection limit, sensitivity, and measuring range are 34 μM (6.5 ppb), 117 nA/mM, and 0.46–66.4 mM for formaldehyde, 9.9 μM (55 ppb), 3.43 μA/mM, and 0.1–30 mM for ethanol, and 0.89 μM (0.36 ppb), 2.4 μA/mM, and 0.01–1 mM for phenol, respectively. Further sensor characteristics such as response time and stability are also determined: t90% (formaldehyde: 4.5 min; ethanol: 69 s; phenol: 27 min), stability at permanent exposure (formaldehyde: 63%, 15 h @ 2.62 mM; ethanol: 86%, 18 h @ 1 mM; phenol: 86%, 16.5 h @ 0.1 mM).

Further data

Item Type: Article in a journal
Refereed: Yes
Institutions of the University: Faculties > Faculty of Engineering Science
Faculties > Faculty of Engineering Science > Chair Functional Materials > Chair Functional Materials - Univ.-Prof. Dr.-Ing. Ralf Moos
Faculties
Faculties > Faculty of Engineering Science > Chair Functional Materials
Profile Fields > Advanced Fields > Advanced Materials
Research Institutions > Research Centres > Bayreuth Center for Material Science and Engineering - BayMAT
Profile Fields
Profile Fields > Advanced Fields
Research Institutions
Research Institutions > Research Centres
Result of work at the UBT: Yes
DDC Subjects: 600 Technology, medicine, applied sciences > 620 Engineering
Date Deposited: 21 Jan 2015 11:09
Last Modified: 12 Apr 2016 06:59
URI: https://eref.uni-bayreuth.de/id/eprint/5666