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Microtubule polyglutamylation is important for regulating cytoskeletal architecture and motility in Trypanosoma brucei

Title data

Jentzsch, Jana ; Sabri, Adal ; Speckner, Konstantin ; Lallinger-Kube, Gertrud ; Weiss, Matthias ; Ersfeld, Klaus:
Microtubule polyglutamylation is important for regulating cytoskeletal architecture and motility in Trypanosoma brucei.
In: Journal of Cell Science. Vol. 133 (15 September 2020) Issue 18 . - jcs248047.
ISSN 1477-9137
DOI: https://doi.org/10.1242/jcs.248047

Abstract in another language

The shape of kinetoplastids, such as , is precisely defined during the stages of the life cycle and governed by a stable subpellicular microtubule cytoskeleton. During the cell cycle and transitions between life cycle stages, this stability has to transiently give way to a dynamic behaviour to enable cell division and morphological rearrangements. How these opposing requirements of the cytoskeleton are regulated is poorly understood. Two possible levels of regulation are activities of cytoskeleton-associated proteins and microtubule post-translational modifications (PTMs). Here, we investigate the functions of two putative tubulin polyglutamylases in , TTLL6A and TTLL12B. Depletion of both proteins leads to a reduction in tubulin polyglutamylation and is associated with disintegration of the posterior cell pole, loss of the microtubule plus-end-binding protein EB1 and alterations of microtubule dynamics. We also observe a reduced polyglutamylation of the flagellar axoneme. Quantitative motility analysis reveals that the PTM imbalance correlates with a transition from directional to diffusive cell movement. These data show that microtubule polyglutamylation has an important role in regulating cytoskeletal architecture and motility in the parasite T. brucei. This article has an associated First Person interview with the first author of the paper.

Further data

Item Type: Article in a journal
Refereed: Yes
Keywords: Cytoskeleton; Microtubules; Post-translational modifications; Trypanosoma brucei
Institutions of the University: Faculties
Faculties > Faculty of Mathematics, Physics und Computer Science > Department of Physics > Chair Experimental Physics I - Physics of Living Matter > Chair Experimental Physics I - Physics of Living Matter - Univ.-Prof. Dr. Matthias Weiss
Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Professor Genetics
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Professor Genetics > Professor Genetics - Univ.-Prof. Dr. Klaus Ersfeld
Faculties > Faculty of Mathematics, Physics und Computer Science
Faculties > Faculty of Mathematics, Physics und Computer Science > Department of Physics
Faculties > Faculty of Mathematics, Physics und Computer Science > Department of Physics > Chair Experimental Physics I - Physics of Living Matter
Result of work at the UBT: Yes
DDC Subjects: 500 Science > 530 Physics
500 Science > 570 Life sciences, biology
Date Deposited: 21 Oct 2020 07:25
Last Modified: 25 Feb 2021 10:55
URI: https://eref.uni-bayreuth.de/id/eprint/58497