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Amphiphilic Graft Copolymers Capable of Mixed-Mode Interaction as Alternative Nonviral Transfection Agents

Title data

Diaz Ariza, Ivonne L. ; Jérôme, Valérie ; Pérez Pérez, León D. ; Freitag, Ruth:
Amphiphilic Graft Copolymers Capable of Mixed-Mode Interaction as Alternative Nonviral Transfection Agents.
In: ACS Applied Bio Materials. Vol. 3 (2021) .
ISSN 2576-6422
DOI: https://doi.org/10.1021/acsabm.0c01123

Official URL: Volltext

Project information

Project financing: National University of Colombia (grant/award number 36061)
Minciencias Colombia (grant/award number 38527)
Upper Franconian Trust (Oberfrankenstiftung, Bayreuth, Germany; grant/award number 03847)

Abstract in another language

Nonviral gene delivery vectors are attractive candidates compared to viral ones due to their lower cytotoxicity and immunogenicity. However, their efficacy still requires improvement. Major challenges are the effective complexation and protection of the DNA cargo and the intracellular dissociation of the polyplexes at the site of action. It is commonly accepted that polymer architecture and chemistry influence polyplex characteristics and have an impact on the transfection mechanism. We developed a library of biocompatible copolymers based on methoxy poly(ethylene glycol) and a hydrophobic block of poly(ε-caprolactone-co-propargyl carbonate) grafted with a predetermined number of poly(2-(dimethylamino)ethyl methacrylate) segments. Such copolymers could efficiently deliver their cargo even in the presence of serum proteins and to various “difficult to transfect” cells, thereby outperforming the current gold standard 25 kDa linear poly(ethylenimine). Statistical correlation analysis shows that an optimization of the transfection in the case of copolymers combining several interactive functions benefits from treatment as a multiparameter problem.

Further data

Item Type: Article in a journal
Refereed: Yes
Keywords: Hydrophobicity; Gene delivery; Genetics; Polymers; Copolymers
Institutions of the University: Faculties > Faculty of Engineering Science > Chair Process Biotechnology > Chair Process Biotechnology - Univ.-Prof. Dr. Ruth Freitag
Result of work at the UBT: Yes
DDC Subjects: 500 Science > 570 Life sciences, biology
600 Technology, medicine, applied sciences
Date Deposited: 19 Jan 2021 09:00
Last Modified: 19 Jan 2021 09:00
URI: https://eref.uni-bayreuth.de/id/eprint/62038