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Reconstructing the Remote Origins of a Fold Singleton from a Flavodoxin-Like Ancestor

Title data

Toledo-Patiño, Saacnicteh ; Chaubey, Manish ; Coles, Murray ; Höcker, Birte:
Reconstructing the Remote Origins of a Fold Singleton from a Flavodoxin-Like Ancestor.
In: Biochemistry. Vol. 58 (December 2019) Issue 48 . - pp. 4790-4793.
ISSN 1520-4995
DOI: https://doi.org/10.1021/acs.biochem.9b00900

Project information

Project title:
Project's official titleProject's id
Protein design from sub-domain sized fragments647548

Project financing: ERC Consolidator Grant

Abstract in another language

Evolutionary processes that led to the emergence of structured protein domains left footprints in the sequences of modern proteins. We searched for such hints employing state-of-the-art sequence analysis and found evidence that the HemD-like fold emerged from the flavodoxin-like fold through segment swap and gene duplication. To verify this hypothesis, we reverted these evolutionary steps experimentally, constructing a HemD-half that resulted in a protein with the canonical flavodoxin-like architecture. These results of fold reconstruction from the sequence of a different fold strongly support our hypothesis of common ancestry. It further illustrates the plasticity of modern proteins to form new folded proteins.

Further data

Item Type: Article in a journal
Refereed: Yes
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Biochemistry > Chair Biochemistry - Univ.-Prof. Dr. Birte Höcker
Result of work at the UBT: Yes
DDC Subjects: 500 Science > 540 Chemistry
500 Science > 570 Life sciences, biology
Date Deposited: 25 Jan 2021 12:50
Last Modified: 25 Jan 2021 13:20
URI: https://eref.uni-bayreuth.de/id/eprint/62335