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Functionalized DNA-spider silk nanohydrogels for controlled protein binding and release

Title data

Humenik, Martin ; Preiß, Tamara ; Gödrich, Sebastian ; Papastavrou, Georg ; Scheibel, Thomas:
Functionalized DNA-spider silk nanohydrogels for controlled protein binding and release.
In: Materials Today Bio. Vol. 6 (March 2020) . - No. 100045.
ISSN 2590-0064
DOI: https://doi.org/10.1016/j.mtbio.2020.100045

Abstract in another language

Hydrogels are excellent scaffolds to accommodate sensitive enzymes in a protective environment. However, the lack of suitable immobilization techniques on substrates and the lack of selectivity to anchor a biocatalyst are major drawbacks preventing the use of hydrogels in bioanalytical devices. Here, nanofilm coatings on surfaces were made of a recombinant spider silk protein (rssp) to induce rssp self-assembly and thus the formation of fibril-based nanohydrogels. To functionalize spider silk nanohydrogels for bioselective binding of proteins, two different antithrombin aptamers were chemically conjugated with the rssp, thereby integrating the target-binding function into the nanohydrogel network. Human thrombin was selected as a sensitive model target, in which the structural integrity determines its activity. The chosen aptamers, which bind various exosites of thrombin, enabled selective and cooperative embedding of the protein into the nanohydrogels. The change of the aptamer secondary structure using complementary DNA sequences led to the release of active thrombin and confirmed the addressable functionalization of spider silk nanohydrogels.

Further data

Item Type: Article in a journal
Refereed: Yes
Keywords: Aptamers; Nanohydrogels; Recombinant spider silk; Self-assembly; Thrombin
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Physical Chemistry II
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Physical Chemistry II > Chair Physical Chemistry II - Univ.-Prof. Dr. Georg Papastavrou
Faculties > Faculty of Engineering Science > Chair Biomaterials > Chair Biomaterials - Univ.-Prof. Dr. Thomas Scheibel
Result of work at the UBT: Yes
DDC Subjects: 500 Science > 540 Chemistry
Date Deposited: 29 Jan 2021 10:36
Last Modified: 29 Jan 2021 10:36
URI: https://eref.uni-bayreuth.de/id/eprint/62593