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From sieve to microscope : An efficient technique for sample transfer in the process of microplastics' quantification

Title data

Prume, Julia A. ; Gorka, Felix ; Löder, Martin G. J.:
From sieve to microscope : An efficient technique for sample transfer in the process of microplastics' quantification.
In: MethodsX. (15 April 2021) . - Art.Nr. 101341.
ISSN 2215-0161
DOI: https://doi.org/10.1016/j.mex.2021.101341

Project information

Project title:
Project's official titleProject's id
BMBF 01BF170501BF1705
SFB 1357 MikroplastikSFB1357

Project financing: Bundesministerium für Bildung und Forschung
Deutsche Forschungsgemeinschaft

Abstract in another language

In the field of microplastics’ quantification, efficient and reproducible methodology is still needed. Procedures of sample fractionation and transfer are often insufficiently reported, although fractionating a sample in similarly sized particles is a crucial prerequisite for the subsequent detection and identification process. At the same time, fractionation is error-prone as particles can be lost during transfer between different vessels. This article presents a four-step technique of sample preparation and microscopic examination, suited for different kind of environmental samples (e.g., water, sediment, soil): The sample is size-fractionated in a sieve cascade (I), rinsed from the sieve and vacuum-filtrated onto a filter (II), rinsed from the filter into a glass petri dish with a low amount of water (III), and examined under the microscope in wet or dry condition (IV). The technique manages on standard laboratory equipment and is reliable for fragments > 300 µm: In a validation experiment with polypropylene, the average recovery was 94 ± 13.5 % (arithmetic mean ± standard deviation) and 100 % (median), respectively.

•Reliable sample transfer after wet-sieving

•Concentration of the pretreated sample in a very small amount of water

•Usage of transmitted light in microscopy

Further data

Item Type: Article in a journal
Refereed: Yes
Keywords: Sample preparation; Wet sieving; Vacuum filtration; Microscopic detection; Method validation
Institutions of the University: Faculties
Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Chair Animal Ecology I
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Chair Animal Ecology I > Chair Animal Ecology I - Univ.-Prof. Dr. Christian Laforsch
Profile Fields
Research Institutions > Research Centres > Bayreuth Center of Ecology and Environmental Research- BayCEER
Research Institutions > Collaborative Research Centers, Research Unit > SFB 1357 - MIKROPLASTIK
Result of work at the UBT: Yes
DDC Subjects: 500 Science > 500 Natural sciences
500 Science > 530 Physics
500 Science > 540 Chemistry
500 Science > 570 Life sciences, biology
Date Deposited: 21 Apr 2021 09:20
Last Modified: 21 Apr 2021 09:20
URI: https://eref.uni-bayreuth.de/id/eprint/64832