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Reproducibility and accuracy of microscale thermophoresis in the NanoTemper Monolith : a multi laboratory benchmark study

Title data

López-Méndez, Blanca ; Baron, Bruno ; Brautigam, Chad A. ; Jowitt, Thomas A. ; Knauer, Stefan H. ; Uebel, Stephan ; Williams, Mark A. ; Sedivy, Arthur ; Abian, Olga ; Abreu, Celeste ; Adamczyk, Malgorzata ; Bal, Wojciech ; Berger, Sylvie ; Buell, Alexander K. ; Carolis, Carlo ; Daviter, Tina ; Fish, Alexander ; Garcia-Alai, Maria ; Guenther, Christian ; Hamacek, Josef ; Hoková, Jitka ; Houser, Josef ; Johnson, Chris ; Kelly, Sharon ; Leech, Andrew ; Mas, Caroline ; Matulis, Daumantas ; McLaughlin, Stephen H. ; Montserret, Roland ; Nasreddine, Rouba ; Nehmé, Reine ; Nguyen, Quyen ; Ortega-Alarcón, David ; Perez, Kathryn ; Pirc, Katja ; Piszczek, Grzegorz ; Podobnik, Marjetka ; Rodrigo, Natalia ; Rokov-Plavec, Jasmina ; Schäfer, Susanne ; Sharpe, Tim ; Southall, June ; Staunton, David ; Tavares, Pedro ; Vanek, Ondrej ; Weyand, Michael ; Wu, Di:
Reproducibility and accuracy of microscale thermophoresis in the NanoTemper Monolith : a multi laboratory benchmark study.
Department of Biological Sciences, BiophysX Centre, Institute of Structural and Molecular Biology, Birkbeck, University of London, Malet Street, London, WC1E 7HX, UK
In: European Biophysics Journal with Biophysics Letters. (April 2021) .
ISSN 1432-1017
DOI: https://doi.org/10.1007/s00249-021-01532-6

Abstract in another language

Microscale thermophoresis (MST), and the closely related Temperature Related Intensity Change (TRIC), are synonyms for a recently developed measurement technique in the field of biophysics to quantify biomolecular interactions, using the (capillary-based) NanoTemper Monolith and (multiwell plate-based) Dianthus instruments. Although this technique has been extensively used within the scientific community due to its low sample consumption, ease of use, and ubiquitous applicability, MST/TRIC has not enjoyed the unambiguous acceptance from biophysicists afforded to other biophysical techniques like isothermal titration calorimetry (ITC) or surface plasmon resonance (SPR). This might be attributed to several facts, e.g., that various (not fully understood) effects are contributing to the signal, that the technique is licensed to only a single instrument developer, NanoTemper Technology, and that its reliability and reproducibility have never been tested independently and systematically. Thus, a working group of ARBRE-MOBIEU has set up a benchmark study on MST/TRIC to assess this technique as a method to characterize biomolecular interactions. Here we present the results of this study involving 32 scientific groups within Europe and two groups from the US, carrying out experiments on 40 Monolith instruments, employing a standard operation procedure and centrally prepared samples. A protein–small molecule interaction, a newly developed protein–protein interaction system and a pure dye were used as test systems. We characterized the instrument properties and evaluated instrument performance, reproducibility, the effect of different analysis tools, the influence of the experimenter during data analysis, and thus the overall reliability of this method.

Further data

Item Type: Article in a journal
Refereed: Yes
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Biopolymers > Chair Biopolymers - Apl. Prof. Dr. Birgitta Wöhrl
Result of work at the UBT: Yes
DDC Subjects: 500 Science > 540 Chemistry
500 Science > 570 Life sciences, biology
Date Deposited: 23 Apr 2021 10:15
Last Modified: 23 Apr 2021 10:15
URI: https://eref.uni-bayreuth.de/id/eprint/64905