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Cell freecirculating DNA in saliva during incremental exercise

Title data

Schmidt, Sebastian:
Cell freecirculating DNA in saliva during incremental exercise.
2015
Event: European College of Sport Science , Wien.
(Conference item: Conference , Speech )

Abstract in another language

Introducion:
Cell free circulating DNA (cfDNA) is an upcoming biomarker in sports physiology. Significant increases of cfDNA have been described in various exercise settings as well as under pathological conditions (Ziegler et al.). Increases of cfDNA can be up to 100-fold above normal resting levels, depending on intensity, severity or duration of the event or stimulus. So far it is unknow whether alterations in blood cfDNA are accompanied by respective changes in saliva.
The aim of this study was to correlate measurements of cfdna in saliva with those in blood during incremental exercise test.

Methods:

10 subjects participated in a standardized step-wise incremental exercise test on a treadmill with 3min intervals and increases of 2 km/h with every step starting at 6 km/h until exhaustion. Capillary blood and saliva samples using the SARSTEDT salivette were taken before and after every step. Quantification of cfDNA in saliva and blood was done by a direct quantitative polymerase chain reaction (qPCR) that does not require DNA purification (Breitbach et al.).

Results:

Resting levels of cfDNA in saliva were on average about 2-fold higher than values in capillary plasma. While increases of cfDNA from rest to exhaustion in capillary plasma were 4,6-fold (p= 0,0001) the concentrations in saliva did not change significantly (1,6-fold, p= 0,651). No significant correlations of cfDNA values from saliva with markers for physical load like blood cfDNA, lactate, heart rate or Borg-values were found. This situation did not improve by correcting the cfDNA values with the respective changes in osmolality of the saliva. IN contrast a medium up to high correlation was found between capillary blood cfDNA concentrations and Borg-values (r= 0.63) or lactate (r= 0.79). Discussion: CfDNA values in saliva were principally measureable but in contrast to values from capillary blood failed to show an immediate response upon physical stress. At rest cfDNA values in saliva were higher than in blood. This could be due to either a physio-logical mechanism of cfDNA release, additional cfDNA sources other than blood or by technical bias involving sample preparation. To enable non-invasive measurement of cfDNA from saliva that reflects physiological alterations these factors need to be studied in more detail

Further data

Item Type: Conference item (Speech)
Refereed: Yes
Institutions of the University: Faculties > Faculty of Law, Business and Economics > Chair Healthcare Management and Health Sciences > Chair Healthcare Management and Health Sciences - Univ.-Prof. Dr. Dr. Dr. h.c. Eckhard Nagel
Result of work at the UBT: No
DDC Subjects: 600 Technology, medicine, applied sciences > 610 Medicine and health
Date Deposited: 18 May 2021 11:48
Last Modified: 18 May 2021 11:48
URI: https://eref.uni-bayreuth.de/id/eprint/65240