on the publication serverTitle data
Yan, Ruiwen ; Kappler, Andreas ; Horn, Marcus A. ; Peiffer, Stefan:
Towards a standardized protocol for studying chemolithoautotrophic denitrification with pyrite at circumneutral pH.
In: Applied Geochemistry.
(10 May 2021)
.
ISSN 0883-2927
DOI: https://doi.org/10.1016/j.apgeochem.2021.104995
Abstract in another language
Laboratory studies on chemolithoautotrophic microbial denitrification coupled to pyrite oxidation at circumneutral pH yielded conflicting results. Some studies indicated that microbial oxidation of pyrite does occur, but several reports have shown that no microbial pyrite oxidation took place in experiments with pyrite as electron donor and nitrate as electron acceptor. We propose that inconsistent experimental and analytical protocols may cause substantial uncertainty in the interpretation of results from such laboratory studies, and may even produce artifacts. In this study, a comprehensive overview of possible pitfalls and artifacts in relation to geochemical and microbiological interferences is provided. Key interferences are impurities of reduced sulfur species associated with pyrite, interferences of nitrite and dissolved Fe(III) with quantitative spectrophotometric determination of Fe(II) and Fe(III) during acidic extractions, interference of oxygen, occurrence of residual iron and sulfur compounds in the reaction medium, and interference of cell-associated and stored sulfur. Therefore, we propose a series of experimental standard protocols to overcome these interferences in future studies on chemolithoautotrophic denitrification with pyrite
Further data
| Item Type: | Article in a journal |
|---|---|
| Refereed: | Yes |
| Keywords: | nitrate reduction; iron oxidation; iron sulfide minerals; geochemical interference; microbiological interference; conflicting results |
| Institutions of the University: | Faculties > Faculty of Biology, Chemistry and Earth Sciences Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Earth Sciences Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Earth Sciences > Chair Hydrology > Chair Hydrology - Univ.-Prof. Dr. Stefan Peiffer Faculties Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Earth Sciences > Chair Hydrology |
| Result of work at the UBT: | Yes |
| DDC Subjects: | 500 Science > 550 Earth sciences, geology |
| Date Deposited: | 19 May 2021 07:08 |
| Last Modified: | 21 Jul 2021 06:05 |
| URI: | https://eref.uni-bayreuth.de/id/eprint/65241 |
