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Towards a standardized protocol for studying chemolithoautotrophic denitrification with pyrite at circumneutral pH

Title data

Yan, Ruiwen ; Kappler, Andreas ; Horn, Marcus A. ; Peiffer, Stefan:
Towards a standardized protocol for studying chemolithoautotrophic denitrification with pyrite at circumneutral pH.
In: Applied Geochemistry. (10 May 2021) .
ISSN 0883-2927
DOI: https://doi.org/10.1016/j.apgeochem.2021.104995

Abstract in another language

Laboratory studies on chemolithoautotrophic microbial denitrification coupled to pyrite oxidation at circumneutral pH yielded conflicting results. Some studies indicated that microbial oxidation of pyrite does occur, but several reports have shown that no microbial pyrite oxidation took place in experiments with pyrite as electron donor and nitrate as electron acceptor. We propose that inconsistent experimental and analytical protocols may cause substantial uncertainty in the interpretation of results from such laboratory studies, and may even produce artifacts. In this study, a comprehensive overview of possible pitfalls and artifacts in relation to geochemical and microbiological interferences is provided. Key interferences are impurities of reduced sulfur species associated with pyrite, interferences of nitrite and dissolved Fe(III) with quantitative spectrophotometric determination of Fe(II) and Fe(III) during acidic extractions, interference of oxygen, occurrence of residual iron and sulfur compounds in the reaction medium, and interference of cell-associated and stored sulfur. Therefore, we propose a series of experimental standard protocols to overcome these interferences in future studies on chemolithoautotrophic denitrification with pyrite

Further data

Item Type: Article in a journal
Refereed: Yes
Keywords: nitrate reduction; iron oxidation; iron sulfide minerals; geochemical interference; microbiological interference; conflicting results
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Earth Sciences > Chair Hydrology > Chair Hydrology - Univ.-Prof. Dr. Stefan Peiffer
Faculties
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Earth Sciences > Chair Hydrology
Result of work at the UBT: Yes
DDC Subjects: 500 Science > 550 Earth sciences, geology
Date Deposited: 19 May 2021 07:08
Last Modified: 21 Jul 2021 06:05
URI: https://eref.uni-bayreuth.de/id/eprint/65241