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A Light-Oxygen-Voltage Receptor Integrates Light and Temperature

Title data

Dietler, Julia ; Schubert, Roman ; Krafft, Tobias G. A. ; Meiler, Simone ; Kainrath, Stephanie ; Richter, Florian ; Schweimer, Kristian ; Weyand, Michael ; Janovjak, Harald ; Möglich, Andreas:
A Light-Oxygen-Voltage Receptor Integrates Light and Temperature.
In: Journal of Molecular Biology. Vol. 433 (July 2021) Issue 15 . - Art.Nr. 167107.
ISSN 0022-2836
DOI: https://doi.org/10.1016/j.jmb.2021.167107

Official URL: Volltext

Project information

Project financing: Alexander von Humboldt-Stiftung
Deutsche Forschungsgemeinschaft

Abstract in another language

Sensory photoreceptors enable organisms to adjust their physiology, behavior, and development in response to light, generally with spatiotemporal acuity and reversibility. These traits underlie the use of photoreceptors as genetically encoded actuators to alter by light the state and properties of heterologous organisms. Subsumed as optogenetics, pertinent approaches enable regulating diverse cellular processes, not least gene expression. Here, we controlled the widely used Tet repressor by coupling to light-oxygen-voltage (LOV) modules that either homodimerize or dissociate under blue light. Repression could thus be elevated or relieved, and consequently protein expression was modulated by light. Strikingly, the homodimeric RsLOV module from Rhodobacter sphaeroides not only dissociated under light but intrinsically reacted to temperature. The limited light responses of wild-type RsLOV at 37 °C were enhanced in two variants that exhibited closely similar photochemistry and structure. One variant improved the weak homodimerization affinity of 40 µM by two-fold and thus also bestowed light sensitivity on a receptor tyrosine kinase. Certain photoreceptors, exemplified by RsLOV, can evidently moonlight as temperature sensors which immediately bears on their application in optogenetics and biotechnology. Properly accounted for, the temperature sensitivity can be leveraged for the construction of signal-responsive cellular circuits.

Further data

Item Type: Article in a journal
Refereed: Yes
Keywords: crystal structure; optogenetics; sensory photoreceptor; signal transduction; Tet repressor
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Biochemistry > Chair Biochemistry - Univ.-Prof. Dr. Andreas Möglich
Research Institutions > Research Centres > Nordbayerisches Zentrum für NMR-Spektroskopie - NMR-Zentrum
Faculties
Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Biochemistry
Research Institutions
Research Institutions > Research Centres
Result of work at the UBT: Yes
DDC Subjects: 500 Science > 570 Life sciences, biology
Date Deposited: 16 Jul 2021 09:23
Last Modified: 16 Jul 2021 09:23
URI: https://eref.uni-bayreuth.de/id/eprint/66559