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Integrative Structural Biology of Protein-RNA Complexes

Title data

Dimitrova-Paternoga, Lyudmila ; Jagtap, Pravin Kumar Ankush ; Chen, Po-Chia ; Hennig, Janosch:
Integrative Structural Biology of Protein-RNA Complexes.
In: Structure. Vol. 28 (2020) Issue 1 . - pp. 6-28.
ISSN 0969-2126
DOI: https://doi.org/10.1016/j.str.2019.11.017

Abstract in another language

Ribonucleoprotein complexes (RNPs) are central to all processes in the cell. One of the prerequisites to understand how RNPs work is to determine their high-resolution structures. With the recent revolution in cryoelectron microscopy this task has become easier for large RNP machines, such as ribosomes, spliceosomes, and polymerases. However, the transient and highly dynamic nature of many RNPs makes structure determination a challenging task. Thus, an integrative structural and molecular biology approach is required, tackling three key challenges: (1) identification of cognate RNA sequences; (2) collection of structural data by conducting X-ray crystallography, NMR, electron microscopy, small-angle scattering (SAS), and other experiments; and (3) the creation of structural models that integrates all experimental restraints. Given the breadth of expertise required, this review presents an overview of available methods and successful examples with the goal to provide readers with a selection of promising options for structure determination of RNPs.

Further data

Item Type: Article in a journal
Refereed: Yes
Keywords: NMR; RNA; RNA binding domain; RNA binding protein; RNA motif identification; RNA recognition; RNP; integrative structural biology; protein-RNA complexes; structural biology; structure modeling
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Biochemistry with an Emphasis on Biophysical Chemistry > Chair Biochemistry with an Emphasis on Biophysical Chemistry - Univ.-Prof. Dr. Janosch Hennig
Result of work at the UBT: No
DDC Subjects: 500 Science > 540 Chemistry
500 Science > 570 Life sciences, biology
Date Deposited: 06 Oct 2021 10:53
Last Modified: 06 Oct 2021 10:53
URI: https://eref.uni-bayreuth.de/id/eprint/67206