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Enhancer RNAs stimulate Pol II pause release by harnessing multivalent interactions to NELF

Title data

Gorbovytska, Vladyslava ; Kim, Seung-Kyoon ; Kuybu, Filiz ; Götze, Michael ; Um, Dahun ; Kang, Keunsoo ; Pittroff, Andreas ; Brennecke, Theresia ; Schneider, Lisa-Marie ; Leitner, Alexander ; Kim, Tae-Kyung ; Kuhn, Claus-D.:
Enhancer RNAs stimulate Pol II pause release by harnessing multivalent interactions to NELF.
In: Nature Communications. Vol. 13 (2022) . - No. 2429.
ISSN 2041-1723

Project information

Project title:
Project's official titleProject's id
Verdrängen eRNAs NELF von RNA polymerase und aktivieren damit die Transkription?No information

Project financing: Deutsche Forschungsgemeinschaft
KU 3514/1-1 und KU 3514/3-1

Abstract in another language

Enhancer RNAs (eRNAs) are long non-coding RNAs that originate from enhancers. Although eRNA transcription is a canonical feature of activated enhancers, the molecular features required for eRNA function and the mechanism of how eRNAs impinge on target gene transcription have not been established. Thus, using eRNA-dependent RNA polymerase II (Pol II) pause release as a model, we examined the requirement of sequence, structure and length of eRNAs for their ability to stimulate Pol II pause release by detaching NELF from paused Pol II. We found eRNA not to exert their function through common structural or sequence motifs. Instead, eRNAs that exhibit a length >200 nucleotides and that contain unpaired guanosines make multiple, allosteric contacts with NELF subunits -A and -E to trigger efficient NELF release. By revealing the molecular determinants of eRNA function, our study establishes eRNAs as an important player in Pol II pause release, and it provides new insight into the regulation of metazoan transcription.

Further data

Item Type: Article in a journal
Refereed: Yes
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Biochemistry VI - RNA Biochemistry > Chair Biochemistry VI - RNA Biochemistry - Univ.-Prof. Dr. Claus-Dieter Kuhn
Profile Fields > Advanced Fields > Molecular Biosciences
Graduate Schools
Graduate Schools > Bayreuth Graduate School of Mathematical and Natural Sciences (BayNAT) > Molecular Biosciences
Graduate Schools > Elite Network Bavaria > Structural Basis of Gene Regulation by Non-coding RNA
Faculties
Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Biochemistry VI - RNA Biochemistry
Profile Fields
Profile Fields > Advanced Fields
Graduate Schools > Bayreuth Graduate School of Mathematical and Natural Sciences (BayNAT)
Graduate Schools > Elite Network Bavaria
Result of work at the UBT: Yes
DDC Subjects: 500 Science > 570 Life sciences, biology
Date Deposited: 28 Feb 2022 07:25
Last Modified: 09 May 2022 12:28
URI: https://eref.uni-bayreuth.de/id/eprint/68803