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Taking advantage of transparency : A proof-of-principle for the analysis of the uptake of labeled microplastic particles by organisms of different functional feeding guilds using an adapted CUBIC protocol

Title data

Ritschar, Sven ; Hüftlein, Frederic ; Schell, Lisa-Marie ; Brehm, Julian ; Laforsch, Christian:
Taking advantage of transparency : A proof-of-principle for the analysis of the uptake of labeled microplastic particles by organisms of different functional feeding guilds using an adapted CUBIC protocol.
In: Science of the Total Environment. Vol. 832 (March 2022) . - No. 154922.
ISSN 0048-9697
DOI: https://doi.org/10.1016/j.scitotenv.2022.154922

Project information

Project title:
Project's official titleProject's id
SFB 1357 Mikroplastik391977956
BayOekotoxNo information

Project financing: Deutsche Forschungsgemeinschaft
Bavarian State Ministry of the Environment and Consumer Protection

Abstract in another language

The analysis of the ingestion of microplastics (MP) by biota is frequently performed through invasive procedures such as chemical digestion protocols or by histological analysis of thin sections. Different, promising approaches for the observation of ingested MP particles pose so called tissue clearing methods. They are currently applied to organs, tissue samples, or whole organisms, rendering the sample transparent and enable to look inside an otherwise opaque environment. To date, there is a lack of methods to detect labeled MP inside an opaque organism's digestive tract without interfering with the sample's integrity. Therefore, our goal was to adapt the CUBIC tissue clearing protocol (Clear, Unobstructed Brain/Body Imaging Cocktails and Computational Analysis) for aquatic and terrestrial organisms of various functional feeding groups for the analysis of the uptake of fluorescent labeled microplastic (MP) particles. We included the buff-tailed bumblebee Bombus terrestris, the compost worm Eisenia fetida, the woodlouse Porcellio scaber, the freshwater shrimp Gammarus roeselii, and the quagga mussel Dreissena bugensis in the analysis. The adapted CUBIC method has led to transparency in all normally opaque organisms. It further offers a simple way of locating fluorescent labeled MP inside the digestive system of the different organisms while leaving them intact.

Further data

Item Type: Article in a journal
Refereed: Yes
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Chair Animal Ecology I > Chair Animal Ecology I - Univ.-Prof. Dr. Christian Laforsch
Research Institutions > Research Centres > Bayreuth Center of Ecology and Environmental Research- BayCEER
Research Institutions > Collaborative Research Centers, Research Unit > SFB 1357 - MIKROPLASTIK
Faculties
Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Chair Animal Ecology I
Research Institutions
Research Institutions > Research Centres
Research Institutions > Collaborative Research Centers, Research Unit
Result of work at the UBT: Yes
DDC Subjects: 500 Science > 570 Life sciences, biology
500 Science > 590 Animals (Zoology)
Date Deposited: 14 Apr 2022 12:10
Last Modified: 29 Apr 2022 10:06
URI: https://eref.uni-bayreuth.de/id/eprint/69215