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Flow Simulation and Gradient Printing of Fluorapatite- and Cell-Loaded Recombinant Spider Silk Hydrogels

Title data

Neubauer, Vanessa J. ; Hüter, Florian ; Wittmann, Johannes ; Trossmann, Vanessa T. ; Kleinschrodt, Claudia ; Alber-Laukant, Bettina ; Rieg, Frank ; Scheibel, Thomas:
Flow Simulation and Gradient Printing of Fluorapatite- and Cell-Loaded Recombinant Spider Silk Hydrogels.
In: Biomolecules. Vol. 12 (2022) Issue 10 . - No. 1413.
ISSN 2218-273X
DOI: https://doi.org/10.3390/biom12101413

Official URL: Volltext

Abstract in another language

Hierarchical structures are abundant in almost all tissues of the human body. Therefore, it is highly important for tissue engineering approaches to mimic such structures if a gain of function of the new tissue is intended. Here, the hierarchical structures of the so-called enthesis, a gradient tissue located between tendon and bone, were in focus. Bridging the mechanical properties from soft to hard secures a perfect force transmission from the muscle to the skeleton upon locomotion. This study aimed at a novel method of bioprinting to generate gradient biomaterial constructs with a focus on the evaluation of the gradient printing process. First, a numerical approach was used to simulate gradient formation by computational flow as a prerequisite for experimental bioprinting of gradients. Then, hydrogels were printed in a single cartridge printing set-up to transfer the findings to biomedically relevant materials. First, composites of recombinant spider silk hydrogels with fluorapatite rods were used to generate mineralized gradients. Then, fibroblasts were encapsulated in the recombinant spider silk-fluorapatite hydrogels and gradually printed using unloaded spider silk hydrogels as the second component. Thereby, adjustable gradient features were achieved, and multimaterial constructs were generated. The process is suitable for the generation of gradient materials, e.g., for tissue engineering applications such as at the tendon/bone interface.

Further data

Item Type: Article in a journal
Refereed: Yes
Keywords: gradient printing; bioprinting; computational fluid dynamics; recombinant spider silk hydrogels; apatite particles; single cartridge set-up; tissue engineering
Institutions of the University: Faculties > Faculty of Engineering Science > Chair Engineering Design and CAD > Chair Engineering Design and CAD - Univ.-Prof. Dr.-Ing Stephan Tremmel
Faculties > Faculty of Engineering Science > Chair Biomaterials
Faculties > Faculty of Engineering Science > Chair Biomaterials > Chair Biomaterials - Univ.-Prof. Dr. Thomas Scheibel
Faculties
Faculties > Faculty of Engineering Science
Faculties > Faculty of Engineering Science > Chair Engineering Design and CAD
Result of work at the UBT: Yes
DDC Subjects: 500 Science > 570 Life sciences, biology
600 Technology, medicine, applied sciences > 610 Medicine and health
600 Technology, medicine, applied sciences > 620 Engineering
Date Deposited: 04 Oct 2022 13:29
Last Modified: 05 Oct 2022 05:58
URI: https://eref.uni-bayreuth.de/id/eprint/72268