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Metabolome analysis of Arabidopsis thaliana roots identifies a key metabolic pathway for iron acquisition

Title data

Schmidt, Holger ; Günther, Carmen ; Weber, Michael ; Spörlein, Cornelia ; Loscher, Sebastian ; Böttcher, Christoph ; Schobert, Rainer ; Clemens, Stephan:
Metabolome analysis of Arabidopsis thaliana roots identifies a key metabolic pathway for iron acquisition.
In: PLoS One. Vol. 9 (2014) Issue 7 . - e102444.
ISSN 1932-6203
DOI: https://doi.org/10.1371/journal.pone.0102444

Official URL: Volltext

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Abstract in another language

Fe deficiency compromises both human health and plant productivity. Thus, it is important to understand plant Fe acquisition strategies for the development of crop plants which are more Fe-efficient under Fe-limited conditions, such as alkaline soils, and have higher Fe density in their edible tissues. Root secretion of phenolic compounds has long been hypothesized to be a component of the reduction strategy of Fe acquisition in non-graminaceous plants. We therefore subjected roots of Arabidopsis thaliana plants grown under Fe-replete and Fe-deplete conditions to comprehensive metabolome analysis by gas chromatography-mass spectrometry and ultra-pressure liquid chromatography electrospray ionization quadrupole time-of-flight mass spectrometry. Scopoletin and other coumarins were found among the metabolites showing the strongest response to two different Fe-limited conditions, the cultivation in Fe-free medium and in medium with an alkaline pH. A coumarin biosynthesis mutant defective in ortho-hydroxylation of cinnamic acids was unable to grow on alkaline soil in the absence of Fe fertilization. Co-cultivation with wild-type plants partially rescued the Fe deficiency phenotype indicating a contribution of extracellular coumarins to Fe solubilization. Indeed, coumarins were detected in root exudates of wild-type plants. Direct infusion mass spectrometry as well as UV/vis spectroscopy indicated that coumarins are acting both as reductants of Fe(III) and as ligands of Fe(II).

Further data

Item Type: Article in a journal
Refereed: Yes
Additional notes: BAYCEER123856
Institutions of the University: Faculties
Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Chair Plant Physiology
Profile Fields
Profile Fields > Advanced Fields
Profile Fields > Advanced Fields > Molecular Biosciences
Profile Fields > Emerging Fields
Profile Fields > Emerging Fields > Food and Health Sciences
Research Institutions > Research Centres > Bayreuth Center of Ecology and Environmental Research- BayCEER
Research Institutions
Research Institutions > Research Centres
Result of work at the UBT: Yes
DDC Subjects: 500 Science
500 Science > 540 Chemistry
500 Science > 570 Life sciences, biology
500 Science > 580 Plants (Botany)
Date Deposited: 25 Mar 2015 08:17
Last Modified: 13 Apr 2016 09:20
URI: https://eref.uni-bayreuth.de/id/eprint/9289