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Root transcript profiling of two Rorippa species reveals gene clusters associated with extreme submergence tolerance

Title data

Sasidharan, Rashmi ; Mustroph, Angelika ; Boonman, Alex ; Akman, Melis ; Ammerlaan, Ankie M. H. ; Breit, Timo ; Schranz, M. Eric ; Voesenek, Laurentius A. C. J. ; van Tienderen, Peter H.:
Root transcript profiling of two Rorippa species reveals gene clusters associated with extreme submergence tolerance.
In: Plant Physiology. Vol. 163 (November 2013) Issue 3 . - pp. 1277-1292.
ISSN 1532-2548
DOI: https://doi.org/10.1104/pp.113.222588

Official URL: Volltext

Abstract in another language

Complete submergence represses photosynthesis and aerobic respiration, causing rapid mortality in most terrestrial plants. However, some plants have evolved traits allowing them to survive prolonged flooding, such as species of the genus Rorippa, close relatives of Arabidopsis (Arabidopsis thaliana). We studied plant survival, changes in carbohydrate and metabolite concentrations, and transcriptome responses to submergence of two species, Rorippa sylvestris and Rorippa amphibia. We exploited the close relationship between Rorippa species and the model species Arabidopsis by using Arabidopsis GeneChip microarrays for whole-genome transcript profiling of roots of young plants exposed to a 24-h submergence treatment or air. A probe mask was used based on hybridization of genomic DNA of both species to the arrays, so that weak probe signals due to Rorippa species/Arabidopsis mismatches were removed. Furthermore, we compared Rorippa species microarray results with those obtained for roots of submerged Arabidopsis plants. Both Rorippa species could tolerate deep submergence, with R. sylvestris surviving much longer than R. amphibia. Submergence resulted in the induction of genes involved in glycolysis and fermentation and the repression of many energy-consuming pathways, similar to the low-oxygen and submergence response of Arabidopsis and rice (Oryza sativa). The qualitative responses of both Rorippa species to submergence appeared roughly similar but differed quantitatively. Notably, glycolysis and fermentation genes and a gene encoding sucrose synthase were more strongly induced in the less tolerant R. amphibia than in R. sylvestris. A comparison with Arabidopsis microarray studies on submerged roots revealed some interesting differences and potential tolerance-related genes in Rorippa species.

Further data

Item Type: Article in a journal
Refereed: Yes
Additional notes: PubMed-ID: 18665916
BAYCEER120210
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Professorship Plant Genetics > Professorship Plant Genetics- Univ.Prof. Dr. Angelika Mustroph
Profile Fields > Advanced Fields > Molecular Biosciences
Research Institutions > Research Centres > Bayreuth Center for Molecular Biosciences - BZMB
Research Institutions > Research Centres > Bayreuth Center of Ecology and Environmental Research- BayCEER
Faculties
Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Professorship Plant Genetics
Profile Fields
Profile Fields > Advanced Fields
Research Institutions
Research Institutions > Research Centres
Result of work at the UBT: No
DDC Subjects: 500 Science > 570 Life sciences, biology
500 Science > 580 Plants (Botany)
Date Deposited: 01 Apr 2015 06:58
Last Modified: 27 Apr 2016 08:51
URI: https://eref.uni-bayreuth.de/id/eprint/9613