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Surface properties of spider silk particles in solution

Title data

Helfricht, Nicolas ; Klug, Maria ; Mark, Andreas ; Kuznetsov, Volodymyr ; Papastavrou, Georg ; Blüm, Claudia ; Scheibel, Thomas:
Surface properties of spider silk particles in solution.
In: Biomaterials Science. Vol. 1 (1 November 2013) Issue 11 . - pp. 1166-1171.
ISSN 2047-4849
DOI: https://doi.org/10.1039/c3bm60109a

Official URL: Volltext

Abstract in another language

Recombinant spider silk proteins, such as eADF4(C16), can be used for various applications. Colloidal particles
of eADF4(C16) show potential as drug delivery systems. Tuning the colloidal properties of suspensions
of eADF4(C16) particles represents a major prerequisite for their use in pharmaceutical
formulations. In this study we determined the surface properties concerning inter-particle interactions by
means of electrophoretic mobility and direct force measurements. The surface charge of eADF4(C16)
spider silk particles was determined as a function of ionic strength and pH, respectively. The resulting
electrophoretic mobility can be described using the O’Brien and White theory and is directly related to
the amino acid sequence of the protein. We determined the extension of a fuzzy protein layer protruding
into the solution by direct force measurements using a colloidal probe technique. This soft layer leads to
deviations in the electrophoretic mobility and is responsible for additional repulsive forces at small
separation distances. These steric forces lead to a stabilization of the particle suspension at high ionic
strength

Further data

Item Type: Article in a journal
Refereed: Yes
Additional notes: Der Artikel wurde zunächst online veröffentlicht am 25.07.2013
Institutions of the University: Faculties
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Physical Chemistry II
Faculties > Faculty of Engineering Science
Faculties > Faculty of Engineering Science > Chair Biomaterials
Faculties > Faculty of Engineering Science > Chair Biomaterials > Chair Biomaterials - Univ.-Prof. Dr. Thomas Scheibel
Faculties > Faculty of Biology, Chemistry and Earth Sciences
Profile Fields > Advanced Fields > Advanced Materials
Profile Fields > Advanced Fields > Molecular Biosciences
Profile Fields > Advanced Fields > Polymer and Colloid Science
Profile Fields > Emerging Fields > Food and Health Sciences
Profile Fields
Profile Fields > Advanced Fields
Profile Fields > Emerging Fields
Result of work at the UBT: Yes
DDC Subjects: 500 Science > 540 Chemistry
600 Technology, medicine, applied sciences > 620 Engineering
Date Deposited: 02 Apr 2015 05:50
Last Modified: 19 May 2017 07:04
URI: https://eref.uni-bayreuth.de/id/eprint/9649