Titlebar

Export bibliographic data
Literature by the same author
plus on the publication server
plus at Google Scholar

 

Influence of Poly(L-Lactic Acid) Nanofibers and BMP-2-Containing Poly(L-Lactic Acid) Nanofibers on Growth and Osteogenic Differentiation of Human Mesenchymal Stem Cells

Title data

Schofer, Markus D. ; Fuchs-Winkelmann, Susanne ; Gräbedünkel, Christian ; Wack, Christina ; Dersch, Roland ; Rudisile, Markus ; Wendorff, Joachim H. ; Greiner, Andreas ; Paletta, Jürgen R. J. ; Boudriot, Ulrich:
Influence of Poly(L-Lactic Acid) Nanofibers and BMP-2-Containing Poly(L-Lactic Acid) Nanofibers on Growth and Osteogenic Differentiation of Human Mesenchymal Stem Cells.
In: The Scientific World Journal. Vol. 8 (2008) . - pp. 1269-1279.
ISSN 1537-744X
DOI: https://doi.org/10.1100/tsw.2008.163

Official URL: Volltext

Abstract in another language

The aim of this study was to characterize synthetic poly-(L-lactic acid) (PLLA) nanofibers concerning their ability to promote growth and osteogenic differentiation of stem cells in vitro, as well as to test their suitability as a carrier system for growth factors. Fiber matrices composed of PLLA or BMP-2-incorporated PLLA were seeded with human mesenchymal stem cells and cultivated over a period of 22 days under growth and osteoinductive conditions, and analyzed during the course of culture, with respect to gene expression of alkaline phosphatase (ALP), osteocalcin (OC), and collagen I (COL-I). Furthermore, COL-I and OC deposition, as well as cell densities and proliferation, were analyzed using fluorescence microscopy. Although the presence of nanofibers diminished the dexamethasone-induced proliferation, there were no differences in cell densities or deposition of either COL-I or OC after 22 days of culture. The gene expression of ALP, OC, and COL-I decreased in the initial phase of cell cultivation on PLLA nanofibers as compared to cover slip control, but normalized during the course of cultivation. The initial down-regulation was not observed when BMP-2 was directly incorporated into PLLA nanofibers by electrospinning, indicating that growth factors like BMP-2 might survive the spinning process in a bioactive form.

Further data

Item Type: Article in a journal
Refereed: Yes
Keywords: nanofibers; tissue engineering; human mesenchymal stem cells; PLLA; BMP
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Macromolecular Chemistry II > Chair Macromolecular Chemistry II - Univ.-Prof. Dr. Andreas Greiner
Faculties
Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Macromolecular Chemistry II
Result of work at the UBT: Yes
DDC Subjects: 500 Science > 540 Chemistry
Date Deposited: 10 Apr 2015 09:38
Last Modified: 10 Apr 2015 09:38
URI: https://eref.uni-bayreuth.de/id/eprint/9662