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Identification of the early postmortem metabolic state of porcine M. semimembranosus using Raman spectroscopy

Title data

Scheier, Rico ; Köhler, Jürgen ; Schmidt, Heinar:
Identification of the early postmortem metabolic state of porcine M. semimembranosus using Raman spectroscopy.
In: Vibrational Spectroscopy. Vol. 70 (January 2014) . - pp. 12-17.
ISSN 0924-2031
DOI: https://doi.org/10.1016/j.vibspec.2013.10.001

Project information

Project title:
Project's official titleProject's id
Grundlagenuntersuchungen zur Raman-Sensorik von Lactat für eine automatisierbare Beurteilung der Fleischqualität in der ProzessketteSchm2724/1-1
Forschungsstelle für Nahrungsmittelqualität (ForN) der Universität Bayreuth am Standort KulmbachNo information

Project financing: Deutsche Forschungsgemeinschaft
Europäische Strukturfonds
EFRE

Abstract in another language

The metabolic conditions in the early postmortem muscle determine meat quality. Raman spectroscopy is shown to follow the early postmortem metabolism (0.5–10 h) of porcine M. semimembranosus (N = 10). To this end the Raman spectra, pH and lactate kinetics were measured in the laboratory. Raman difference spectra were utilized to identify the spectral changes and to assign them to metabolic and structural alterations in the pre-rigor (50–120 min) and rigor (2–8.5 h) phases. In the Raman spectra, the decreasing pH was indicated by three signals assigned to phosphorylated metabolites and inorganic phosphate. Furthermore, the degradation of glycogen to lactate and a reduction of signal intensity of α-helical proteins were revealed. In the pre-rigor phase, degradation of phosphocreatine to creatine and reduction of oxy- to deoxymyoglobin was found. In the rigor phase, additionally, degradation of ATP to inosine monophosphate (IMP) was observed. Good agreement was achieved between measured and simulated Raman difference spectra. In the pre-rigor and rigor time frame, normal and deviating meat quality could be distinguished based on signals of phosphocreatine, ATP, IMP and α-helical proteins. This work provides a deeper understanding and the first semi-quantitative description of the early postmortem Raman spectra of meat which show potential for the non-invasive and early detection of the metabolic state of meat, and hence for the identification of deviating meat qualities.

Further data

Item Type: Article in a journal
Refereed: Yes
Keywords: Energy; metabolism; In situ; pH; Lactate; ATP; IMP
Institutions of the University: Faculties
Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Lehrstuhl Bioanalytik und Lebensmittelanalytik
Profile Fields
Profile Fields > Emerging Fields
Profile Fields > Emerging Fields > Food and Health Sciences
Research Institutions
Research Institutions > Research Units
Research Institutions > Research Units > Forschungsstelle für Nahrungsmittelqualität - ForN
Result of work at the UBT: Yes
DDC Subjects: 500 Science
500 Science > 570 Life sciences, biology
Date Deposited: 01 Apr 2015 09:16
Last Modified: 28 Jan 2016 11:13
URI: https://eref.uni-bayreuth.de/id/eprint/9726