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Energetic communication between functional sites of the gene-3-protein during infection by phage fd

Title data

Hoffmann-Thoms, Stephanie ; Jakob, Roman P. ; Schmid, Franz X.:
Energetic communication between functional sites of the gene-3-protein during infection by phage fd.
In: Journal of Molecular Biology. Vol. 426 (17 April 2014) Issue 8 . - pp. 1711-1722.
ISSN 0022-2836
DOI: https://doi.org/10.1016/j.jmb.2014.01.002

Abstract in another language

To initiate infection of Escherichia coli, phage fd uses its gene-3-protein (G3P) to bind first to an F pilus and then to the TolA protein at the cell surface. G3P is normally auto-inhibited because a tight interaction between the two N-terminal domains N1 and N2 buries the TolA binding site. Binding of N2 to the pilus activates G3P by initiating long-range conformational changes that are relayed to the domain interface and to a proline timer. We discovered that the 23-28 loop of the N1 domain is critical for propagating these conformational signals. The analysis of the stability and the folding dynamics of G3P variants with a shortened loop combined with TolA interaction studies and phage infection experiments reveal how the contact between the N2 domain and the 23-28 loop of N1 is energetically linked with the interdomain region and the proline timer and how it affects phage infectivity. Our results illustrate how conformational transitions and prolyl cis/trans isomerization can be coupled energetically and how conformational signals to and from prolines can be propagated over long distances in proteins.

Further data

Item Type: Article in a journal
Refereed: Yes
Additional notes: PubMed-ID: 24440124
Institutions of the University: Faculties
Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Former Professors > Professorship Biochemistry - Univ.-Prof. Dr. Franz Xaver Schmid
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Professorship Biochemistry
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Former Professors
Result of work at the UBT: Yes
DDC Subjects: 500 Science > 540 Chemistry
Date Deposited: 21 Apr 2015 13:31
Last Modified: 22 Apr 2015 07:11
URI: https://eref.uni-bayreuth.de/id/eprint/10475