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Initiation of phage infection by partial unfolding and prolyl isomerization

Title data

Hoffmann-Thoms, Stephanie ; Weininger, Ulrich ; Eckert, Barbara ; Jakob, Roman P. ; Koch, Johanna R. ; Balbach, Jochen ; Schmid, Franz X.:
Initiation of phage infection by partial unfolding and prolyl isomerization.
In: The Journal of Biological Chemistry. Vol. 288 (3 May 2013) Issue 18 . - pp. 12979-12991.
ISSN 1083-351X
DOI: https://doi.org/10.1074/jbc.M112.442525

Abstract in another language

Infection of Escherichia coli by the filamentous phage fd starts with the binding of the N2 domain of the phage gene-3-protein to an F pilus. This interaction triggers partial unfolding of the gene-3-protein, cis → trans isomerization at Pro-213, and domain disassembly, thereby exposing its binding site for the ultimate receptor TolA. The trans-proline sets a molecular timer to maintain the binding-active state long enough for the phage to interact with TolA. We elucidated the changes in structure and local stability that lead to partial unfolding and thus to the activation of the gene-3-protein for phage infection. Protein folding and TolA binding experiments were combined with real-time NMR spectroscopy, amide hydrogen exchange measurements, and phage infectivity assays. In combination, the results provide a molecular picture of how a local unfolding reaction couples with prolyl isomerization not only to generate the activated state of a protein but also to maintain it for an extended time.

Further data

Item Type: Article in a journal
Refereed: Yes
Additional notes: PubMed-ID: 17822712
Institutions of the University: Faculties
Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Former Professors > Professorship Biochemistry - Univ.-Prof. Dr. Franz Xaver Schmid
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Professorship Biochemistry
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Former Professors
Result of work at the UBT: Yes
DDC Subjects: 500 Science > 540 Chemistry
Date Deposited: 21 Apr 2015 13:48
Last Modified: 22 Apr 2015 07:15
URI: https://eref.uni-bayreuth.de/id/eprint/10537