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Reprogramming the infection mechanism of a filamentous phage.

Title data

Lorenz, Stefan H. ; Schmid, Franz X.:
Reprogramming the infection mechanism of a filamentous phage.
In: Molecular microbiology. Vol. 80 (May 2011) Issue 3 . - pp. 827-834.
ISSN 1365-2958
DOI: https://doi.org/10.1111/j.1365-2958.2011.07617.x

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Abstract in another language

When they infect Escherichia coli cells, the filamentous phages IF1 and fd first interact with a pilus and then target TolA as their common receptor. They use the domains N2 and N1 of their gene-3-proteins (G3P) for these interactions but differ in the mechanism of infection. In G3P of phage IF1, N1 and N2 are independent modules that are permanently binding-active. G3P of phage fd is usually in a closed state in which N1 and N2 are tightly associated. The TolA binding site is thus inaccessible and the phage incompetent for infection. Partial unfolding and prolyl isomerization must occur to abolish the domain interactions and expose the TolA binding site. This complex mechanism of phage fd could be changed to the simple infection mechanism of phage IF1 by reprogramming its G3P following physicochemical rules of protein stability. The redesigned phage fd was robust and as infectious as wild-type phage fd.

Further data

Item Type: Article in a journal
Refereed: Yes
Additional notes: PubMed-ID: 21392130
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Former Professors > Professorship Biochemistry - Univ.-Prof. Dr. Franz Xaver Schmid
Faculties
Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Professorship Biochemistry
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Former Professors
Result of work at the UBT: Yes
DDC Subjects: 500 Science > 540 Chemistry
Date Deposited: 22 Apr 2015 08:24
Last Modified: 22 Apr 2015 08:24
URI: https://eref.uni-bayreuth.de/id/eprint/10552