Titlebar

Export bibliographic data
Literature by the same author
plus on the publication server
plus at Google Scholar

 

The hsp70 chaperone DnaK is a secondary amide peptide bond cis-trans isomerase

Title data

Schiene-Fischer, Cordelia ; Habazettl, Judith ; Schmid, Franz X. ; Fischer, Gunter:
The hsp70 chaperone DnaK is a secondary amide peptide bond cis-trans isomerase.
In: Nature Structural & Molecular Biology. Vol. 9 (June 2002) Issue 6 . - pp. 419-424.
ISSN 1545-9985
DOI: https://doi.org/10.1038/nsb804

Official URL: Volltext

Related URLs

Abstract in another language

Peptidyl prolyl cis-trans isomerases can enzymatically assist protein folding, but these enzymes exclusively target the peptide bond preceding proline residues. Here we report the identification of the Hsp70 chaperone DnaK as the first member of a novel enzyme class of secondary amide peptide bond cis-trans isomerases (APIases). APIases selectively accelerate the cis-trans isomerization of nonprolyl peptide bonds. Results from independent experiments support the APIase activity of DnaK: (i) exchange crosspeaks between the cis-trans conformers appear in 2D (1)H NMR exchange spectra of oligopeptides (ii) the rate constants for the cis-trans isomerization of various dipeptides increase and (iii) refolding of the RNase T1 P39A variant is catalyzed. The APIase activity shows both regio and stereo selectivity and is stimulated two-fold in the presence of the complete DnaK/GrpE/DnaJ/ATP refolding system. Moreover, known DnaK-binding oligopeptides simultaneously affect the APIase activity of DnaK and the refolding yield of denatured firefly luciferase in the presence of DnaK/GrpE/DnaJ/ATP. These results suggest a new role for the chaperone as a regioselective catalyst for bond rotation in polypeptides.

Further data

Item Type: Article in a journal
Refereed: Yes
Additional notes: PubMed-ID: 12032550
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Former Professors > Professorship Biochemistry - Univ.-Prof. Dr. Franz Xaver Schmid
Faculties
Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Professorship Biochemistry
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Former Professors
Result of work at the UBT: Yes
DDC Subjects: 500 Science > 540 Chemistry
Date Deposited: 27 Apr 2015 09:50
Last Modified: 27 Apr 2015 09:50
URI: https://eref.uni-bayreuth.de/id/eprint/10847