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Reassessment of the putative chaperone function of prolyl-cis/trans-isomerases

Title data

Kern, Gunther ; Kern, Dorothee ; Schmid, Franz X. ; Fischer, Gunter:
Reassessment of the putative chaperone function of prolyl-cis/trans-isomerases.
In: FEBS Letters. Vol. 348 (11 July 1994) Issue 2 . - pp. 145-148.
ISSN 1873-3468
DOI: https://doi.org/10.1016/0014-5793(94)00591-5

Abstract in another language

The folding of proteins can be assisted by two unrelated groups of helper molecules. Chaperones suppress non-productive side reactions by stoichiometric binding to folding intermediates, and folding enzymes catalyze slow rate-limiting steps of folding. We reinvestigated, whether peptidyl-prolyl-cis/trans-isomerases of the cyclophilin type act simultaneously as chaperones and as folding catalysts in the reactivation of human carbonic anhydrase II, as reported recently [Freskgård, P.-O. et al. (1992) Science 258, 466-468; Rinfret, A. et al. (1994) Biochemistry 33, 1668-1673]. No increase in the yield of native carbonic anhydrase-II could be detected in the presence of three different prolyl isomerases, when reactivation was followed by a sensitive assay for an extended time of 4 h. We conclude that the role of prolyl isomerases in the refolding of carbonic anhydrase can be explained solely by their isomerase activity. There is no need to invoke simultaneous functions as chaperones for these folding catalysts.

Further data

Item Type: Article in a journal
Refereed: Yes
Additional notes: PubMed-ID: 7913447
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Former Professors > Professorship Biochemistry - Univ.-Prof. Dr. Franz Xaver Schmid
Faculties
Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Professorship Biochemistry
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Former Professors
Result of work at the UBT: No
DDC Subjects: 500 Science > 540 Chemistry
Date Deposited: 15 May 2015 09:27
Last Modified: 11 Mar 2016 10:44
URI: https://eref.uni-bayreuth.de/id/eprint/13509