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The family of cold shock proteins of Bacillus subtilis : Stability and dynamics in vitro and in vivo

Title data

Schindler, Thomas ; Graumann, Peter L. ; Perl, Dieter ; Ma, Saufung ; Schmid, Franz X. ; Marahiel, Mohamed A.:
The family of cold shock proteins of Bacillus subtilis : Stability and dynamics in vitro and in vivo.
In: The Journal of Biological Chemistry. Vol. 274 (5 February 1999) Issue 6 . - pp. 3407-3413.
ISSN 1083-351X
DOI: https://doi.org/10.1074/jbc.274.6.3407

Abstract in another language

Bacillus subtilis possesses three homologous small cold shock proteins (CSPs; CspB, CspC, CspD, sequence identity >72%). They share a similar beta-sheet structure, as shown by circular dichroism, and have a very low conformational stability, with CspC being the least stable. Similar to CspB, CspC and CspD unfold and refold extremely fast in a N <==> U two-state reaction with average lifetimes of only 100-150 ms for the native state and 1-6 ms for the unfolded states at 25 degreesC. As a consequence of their low stability and low kinetic protection against unfolding, all three cold shock proteins are rapidly degraded by proteases in vitro. Analysis of the CSP stabilities in vivo by pulse-chase experiments revealed that CspB and CspD are stable during logarithmic growth at 37 degreesC as well as after cold shock. The cellular half-life of CspC is shortened at 37 degreesC, but under cold shock conditions CspC becomes stable. The proteolytic susceptibility of the CSPs in vitro was strongly reduced in the presence of a nucleic acid ligand, suggesting that the observed stabilization of CSPs in vivo is mediated by binding to their substrate mRNA at 37 degreesC and, in particular, under cold shock conditions.

Further data

Item Type: Article in a journal
Refereed: Yes
Additional notes: PubMed-ID: 9920884
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Former Professors > Professorship Biochemistry - Univ.-Prof. Dr. Franz Xaver Schmid
Faculties
Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Professorship Biochemistry
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Former Professors
Result of work at the UBT: Yes
DDC Subjects: 500 Science > 540 Chemistry
Date Deposited: 27 May 2015 06:55
Last Modified: 27 May 2015 06:55
URI: https://eref.uni-bayreuth.de/id/eprint/14334