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Single-cell genomics of uncultivated deep-branching magnetotactic bacteria reveals a conserved set of magnetosome genes

Title data

Kolinko, Sebastian ; Richter, Michael ; Glöckner, Frank-Oliver ; Brachmann, Andreas ; Schüler, Dirk:
Single-cell genomics of uncultivated deep-branching magnetotactic bacteria reveals a conserved set of magnetosome genes.
In: Environmental Microbiology. (2015) .
ISSN 1758-2229
DOI: https://doi.org/10.1111/1462-2920.12907

Abstract in another language

While magnetosome biosynthesis within the magnetotactic Proteobacteria is increasingly well understood, much less is known about the genetic control within deep-branching phyla which have a unique ultrastructure and biosynthesize up to several hundreds of bullet-shaped magnetite magnetosomes arranged in multiple bundles of chains, but have no cultured representatives. Recent metagenomic analysis identified magnetosome genes in the genus ′Candidatus Magnetobacterium′ homologous to those in Proteobacteria. However, metagenomic analysis has been limited to highly abundant members of the community and therefore, only little is known about the magnetosome biosynthesis, ecophysiology and metabolic capacity in deep-branching MTB.

Here we report the analysis of single-cell derived draft genomes of three deep-branching uncultivated MTB. Single-cell sorting followed by WGA generated draft genomes of Candidatus Magnetobacterium bavaricum and Candidatus Magnetoovum chiemensis CS-04 of the Nitrospirae phylum. Furthermore, we present the first, nearly complete draft genome of a magnetotactic representative from the candidate phylum Omnitrophica, tentatively named Candidatus Omnitrophus magneticus SKK-01. Besides key metabolic features consistent with a common chemolithoautotrophic lifestyle, we identified numerous, partly novel genes most likely involved in magnetosome biosynthesis of bullet-shaped magnetosomes and their arrangement in multiple bundles of chains.

Further data

Item Type: Article in a journal
Refereed: Yes
Additional notes: Accepted Article = Accepted, unedited article published online and citable. The final edited and typeset version of record will appear in future
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Chair Microbiology > Chair Microbiology - Univ.-Prof. Dr. Dirk Schüler
Faculties
Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Chair Microbiology
Result of work at the UBT: Yes
DDC Subjects: 500 Science
500 Science > 570 Life sciences, biology
Date Deposited: 16 Jul 2015 14:12
Last Modified: 10 Mar 2016 13:48
URI: https://eref.uni-bayreuth.de/id/eprint/16711