Titlebar

Export bibliographic data
Literature by the same author
plus on the publication server
plus at Google Scholar

 

Interaction of MDM33 with mitochondrial inner membrane homeostasis pathways in yeast

Title data

Klecker, Till ; Wemmer, Megan ; Haag, Mathias ; Weig, Alfons ; Böckler, Stefan ; Langer, Thomas ; Nunnari, Jodi ; Westermann, Benedikt:
Interaction of MDM33 with mitochondrial inner membrane homeostasis pathways in yeast.
Universität Bayreuth, Zellbiologie, 95440 Bayreuth, Germany; University of California Davis, Department of Molecular and Cellular Biology, Davis, CA 95616, USA; Universität zu Köln, Institut für Genetik, 50931 Köln, Germany; Universität Bayreuth, DNA Analytik, 95440 Bayreuth, Germany
In: Scientific Reports. Vol. 5 (2015) . - Article Number 18344.
ISSN 2045-2322
DOI: https://doi.org/10.1038/srep18344

Project information

Project title:
Project's official titleProject's id
Open Access PublizierenNo information

Project financing: Deutsche Forschungsgemeinschaft

Abstract in another language

Membrane homeostasis affects mitochondrial dynamics, morphology, and function. Here we report genetic and proteomic data that reveal multiple interactions of Mdm33, a protein essential for normal mitochondrial structure, with components of phospholipid metabolism and mitochondrial inner membrane homeostasis. We screened for suppressors of MDM33 overexpression-induced growth arrest and isolated binding partners by immunoprecipitation of cross-linked cell extracts. These approaches revealed genetic and proteomic interactions of Mdm33 with prohibitins, Phb1 and Phb2, which are key components of mitochondrial inner membrane homeostasis. Lipid profiling by mass spectrometry of mitochondria isolated from Mdm33-overexpressing cells revealed that high levels of Mdm33 affect the levels of phosphatidylethanolamine and cardiolipin, the two key inner membrane phospholipids. Furthermore, we show that cells lacking Mdm33 show strongly decreased mitochondrial fission activity indicating that Mdm33 is critical for mitochondrial membrane dynamics. Our data suggest that MDM33 functionally interacts with components important for inner membrane homeostasis and thereby supports mitochondrial division.

Further data

Item Type: Article in a journal
Refereed: Yes
Additional notes: BAYCEER133211
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Professorship Cell Biology > Professorship Cell Biology - Univ.-Prof. Dr. Benedikt Westermann
Faculties
Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Professorship Cell Biology
Result of work at the UBT: Yes
DDC Subjects: 500 Science > 570 Life sciences, biology
Date Deposited: 08 Jun 2016 09:07
Last Modified: 26 Jan 2017 11:57
URI: https://eref.uni-bayreuth.de/id/eprint/32555