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Genetically engineered organization : protein template, biological recognition sites and nanoparticles

Title data

Jehle, Franziska ; Valverde-Tercedor, Carmen ; Reichel, Victoria ; Carillo, Maria A. ; Bennet, Mathieu ; Günther, Erika ; Wirth, Richard ; Mickoleit, Frank ; Zarivach, Raz ; Schüler, Dirk ; Blank, Kerstin G. ; Faivre, Damien:
Genetically engineered organization : protein template, biological recognition sites and nanoparticles.
In: Advanced Materials Interfaces. Vol. 3 (28 June 2016) .
ISSN 2196-7350
DOI: https://doi.org/10.1002/admi.201600285

Project information

Project financing: Deutsche Forschungsgemeinschaft

Abstract in another language

Nanoparticles and their assemblies exhibit properties that can be used for a wide range of applications. However, creating multifunctional assemblies has remained challenging. Inspired by magnetotactic bacteria, genetically engineered single building blocks from magnetosome chains are used and complemented by additional components to form fluorescent assemblies of nanoparticles of varying types. This strategy is illustrated by the use of a protein from magnetotactic bacteria (MamK) known to form filaments in vivo and in vitro. A fusion protein of MamK and the fluorescent protein mCherry is recombinantly expressed and isolated using a hexahistidine tag that is subsequently used to bind functionalized gold nanoparticles to polymerized MamK_mCherry_His6 filaments. The versatility of this modular approach is further exemplified by the concomitant addition of biological or synthetic magnetic nanoparticles functionalized with a nanobody directed against mCherry. The as-formed structures are fluorescent and can be actuated by an external magnetic field. This study shows again how nature's strategies can be applied for designing multifunctional materials.

Further data

Item Type: Article in a journal
Refereed: Yes
Keywords: biomimetic chains; magnetic nanoparticles; magnetosomes; magnetotactic bacteria; MamK
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Chair Microbiology > Chair Microbiology - Univ.-Prof. Dr. Dirk Schüler
Faculties
Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Chair Microbiology
Result of work at the UBT: Yes
DDC Subjects: 500 Science
500 Science > 570 Life sciences, biology
Date Deposited: 01 Dec 2016 13:08
Last Modified: 01 Dec 2016 13:08
URI: https://eref.uni-bayreuth.de/id/eprint/35394