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Size Sorting of Protein Assemblies Using Polymeric Gradient Surfaces

Title data

Ionov, Leonid ; Stamm, Manfred ; Diez, Stefan:
Size Sorting of Protein Assemblies Using Polymeric Gradient Surfaces.
In: Nano Letters. Vol. 5 (1 October 2005) Issue 10 . - pp. 1910-1914.
ISSN 1530-6992
DOI: https://doi.org/10.1021/nl051235h

Abstract in another language

We report on a novel approach for the size-dependent fractionation of protein assemblies on polymeric surfaces. Using a simple temperature gradient method to generate one-dimensional gradients of grafted poly(ethylene glycol), we fabricated silicon-oxide chips with a gradually changing surface density of kinesin motor molecules. We demonstrate that such a bioactive surface can be used to sort gliding microtubules according to their length. To our knowledge, this is the first example of the self-organized sorting of protein assemblies on surfaces.We report on a novel approach for the size-dependent fractionation of protein assemblies on polymeric surfaces. Using a simple temperature gradient method to generate one-dimensional gradients of grafted poly(ethylene glycol), we fabricated silicon-oxide chips with a gradually changing surface density of kinesin motor molecules. We demonstrate that such a bioactive surface can be used to sort gliding microtubules according to their length. To our knowledge, this is the first example of the self-organized sorting of protein assemblies on surfaces.

Further data

Item Type: Article in a journal
Refereed: Yes
Institutions of the University: Faculties > Faculty of Engineering Science > Professur Biofabrikation > Professur Biofabrikation - Univ.-Prof. Dr. Leonid Ionov
Faculties
Faculties > Faculty of Engineering Science
Faculties > Faculty of Engineering Science > Professur Biofabrikation
Result of work at the UBT: Yes
DDC Subjects: 500 Science > 540 Chemistry
Date Deposited: 22 Jun 2017 15:05
Last Modified: 22 Jun 2017 15:05
URI: https://eref.uni-bayreuth.de/id/eprint/37760