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Artificial microniches for probing mesenchymal stem cell fate in 3D

Title data

Ma, Yujie ; Neubauer, Martin Peter ; Thiele, Julian ; Fery, Andreas ; Huck, Wilhelm T. S.:
Artificial microniches for probing mesenchymal stem cell fate in 3D.
In: Biomaterials Science. Vol. 2 (June 2014) Issue 11 . - pp. 1661-1671.
ISSN 2047-4849
DOI: https://doi.org/10.1039/C4BM00104D

Official URL: Volltext

Abstract in another language

Droplet microfluidics is combined with bio-orthogonal thiol--ene click chemistry to fabricate micrometer-sized, monodisperse fibrinogen-containing hyaluronic acid hydrogel microbeads in a mild, radical-free procedure in the presence of human mesenchymal stem cells (hMSCs). The gel beads serve as microniches for the 3D culture of single hMSCs, containing hyaluronic acid and additional fibrinogen for cell surface binding, and they are porous and stable in tissue culture medium for up to 4 weeks with mechanical properties right in the range of soft solid tissues (0.9--9.2 kPa). The encapsulation procedure results in 70 viable hMSCs in the microbeads after 24 hours of culture and a very high degree of viability of the cells after long term culture of 2 weeks. hMSCs embedded in the microniches display an overall rounded morphology, consistent with those previously observed in 3D culture. Upon induction, the multi- potency and differentiation potential of the hMSCs are characterized by staining of corresponding bio- markers, demonstrating a clear heterogeneity in the cell population. These hydrogel microbeads represent a versatile microstructured material platform with great potential for studying the differences of material cues and soluble factors in stem cell differentiation in a 3D tissue-like environment at the single cell level.

Further data

Item Type: Article in a journal
Refereed: Yes
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Former Professors > Chair Physical Chemistry II - Univ.-Prof. Dr. Andreas Fery
Faculties
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Physical Chemistry II
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Former Professors
Result of work at the UBT: Yes
DDC Subjects: 500 Science > 500 Natural sciences
500 Science > 530 Physics
500 Science > 540 Chemistry
Date Deposited: 05 Dec 2014 09:39
Last Modified: 12 Feb 2019 09:50
URI: https://eref.uni-bayreuth.de/id/eprint/3924