Titlebar

Export bibliographic data
Literature by the same author
plus on the publication server
plus at Google Scholar

 

Thermoresponsive PEG-Based Polymer Layers : Surface Characterization with AFM Force Measurements

Title data

Kessel, Stefanie ; Schmidt, Stephan ; Müller, Renate ; Wischerhoff, Erik ; Laschewsky, Andre ; Lutz, Jean-François ; Uhlig, Katja ; Lankenau, Andreas ; Duschl, Claus ; Fery, Andreas:
Thermoresponsive PEG-Based Polymer Layers : Surface Characterization with AFM Force Measurements.
In: Langmuir. Vol. 26 (2010) Issue 5 . - pp. 3462-3467.
ISSN 1520-5827
DOI: https://doi.org/10.1021/la903007v

Official URL: Volltext

Abstract in another language

Thermoresponsive polymer-coated surfaces based on poly(2-(2-methoxyethoxy)ethyl methacrylate-co-oliog(ethylene glycol) methacrylate) P(MEO(2)MA-co-OEGMA) allow switching between cell attachment and detachment. Here, we investigate the temperature-dependent surface interactions between the polymer coating and a colloidal probe in an aqueous medium by means of atomic force microscopy (AFM) force-distance measurements. The analysis of the adhesion forces from AFM retraction curves indentifies two kinds of regimes for the copolymer at temperatures below and above the lower critical solution temperature (LCST). Whereas at 25 degrees C the surface interactions with the polymer in the swollen state are dominated by repulsive forces, at 37 degrees C the surface interactions switch to attractive forces and a stronger adhesion is detected by AFM. Running several healing/cooling cycles repeatedly shows that switching the surface properties provides reproducible adhesion force values. Time-dependent measurements give insight into the switching kinetics, demonstrating that the cell response is coupled to the polymer kinetics but probably limited by the cellular rearrangements.

Further data

Item Type: Article in a journal
Refereed: Yes
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Former Professors > Chair Physical Chemistry II - Univ.-Prof. Dr. Andreas Fery
Faculties
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Physical Chemistry II
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Former Professors
Result of work at the UBT: Yes
DDC Subjects: 500 Science > 500 Natural sciences
500 Science > 530 Physics
500 Science > 540 Chemistry
Date Deposited: 08 Dec 2014 10:45
Last Modified: 10 Apr 2018 12:45
URI: https://eref.uni-bayreuth.de/id/eprint/3984