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A multipurpose modular system for high-resolution microscopy at high hydrostatic pressure

Title data

Vass, Hugh ; Black, S. Lucas ; Herzig, Eva M. ; Ward, F. Bruce ; Clegg, Paul S. ; Allen, Rosalind J.:
A multipurpose modular system for high-resolution microscopy at high hydrostatic pressure.
In: Review of Scientific Instruments. Vol. 81 (May 2010) Issue 5 .
ISSN 1089-7623
DOI: https://doi.org/10.1063/1.3427224

Abstract in another language

We have developed a modular system for high-resolution microscopy at high hydrostatic pressure. The system consists of a pressurized cell of volume ∼100 μl, a temperature controlled holder, a ram, and a piston. We have made each of these components in several versions which can be interchanged to allow a wide range of applications. Here, we report two pressure cells with pressure ranges 0.1–700 MPa and 0.1–100 MPa, which can be combined with hollow or solid rams and pistons. Our system is designed to work with fluorescent samples (using a confocal or epifluorescence microscope), but also allows for transmitted light microscopy via the hollow ram and piston. The system allows precise control of pressure and temperature (−20 to 70 °C), as well as rapid pressure quenching. We demonstrate its performance and versatility with two applications: time-resolved imaging of colloidal phase transitions caused by pressure changes between 0.1 and 100 MPa, and imaging the growth of Escherichia coli bacteria at 50 MPa. We also show that the isotropic-nematic phase transition of pentyl-cyanobiphenyl (5CB) liquid crystal provides a simple, convenient, and accurate method for calibrating pressure in the range 0.1–200 MPa

Further data

Item Type: Article in a journal
Refereed: Yes
Institutions of the University: Faculties > Faculty of Mathematics, Physics und Computer Science > Department of Physics
Faculties > Faculty of Mathematics, Physics und Computer Science > Department of Physics > Juniorprofessur Experimentalphysik VII - Dynamik und Strukturbildung > Juniorprofessur Experimentalphysik VII - Dynamik und Strukturbildung - Juniorprof. Dr. Eva Maria Herzig
Faculties
Faculties > Faculty of Mathematics, Physics und Computer Science
Faculties > Faculty of Mathematics, Physics und Computer Science > Department of Physics > Juniorprofessur Experimentalphysik VII - Dynamik und Strukturbildung
Result of work at the UBT: No
DDC Subjects: 500 Science > 530 Physics
Date Deposited: 06 Oct 2017 09:28
Last Modified: 06 Oct 2017 09:28
URI: https://eref.uni-bayreuth.de/id/eprint/39917