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Force Generation of Curved Actin Gels Characterized by Combined AFM-Epifluorescence Measurements

Title data

Schmidt, Stephan ; Helfer, Emmanuele ; Carlier, Marie-France ; Fery, Andreas:
Force Generation of Curved Actin Gels Characterized by Combined AFM-Epifluorescence Measurements.
In: Biophysical Journal. Vol. 98 (19 May 2010) Issue 10 . - pp. 2246-2253.
ISSN 1542-0086
DOI: https://doi.org/10.1016/j.bpj.2010.01.055

Official URL: Volltext

Abstract in another language

Polymerization of actin into branched filaments is the driving force behind active migration of eukaryotic cells and motility of intracellular organelles. The site-directed assembly of a polarized branched array forms an expanding gel that generates the force that pushes the membrane. Here, we use atomic force microscopy to understand the relation between actin polymerization and the produced force. Functionalized spherical colloidal probes of varying size and curvature are attached to the atomic force microscopy cantilever and initiate the formation of a polarized actin gel in a solution mimicking the in vivo context. The gel growth is recorded by epifluorescence microscopy both against the cantilever and in the perpendicular (lateral) nonconstrained direction. In this configuration, the gel growth stops simultaneously in both directions at the stall force, which corresponds to a pressure of 0.15 nN/mu m(2). The results show that the growth of the gel is limited laterally, in the absence of external force, by internal mechanical stresses resulting from a combination of the curved geometry and the molecular mechanism of site-directed assembly of a cohesive branched filament array.

Further data

Item Type: Article in a journal
Refereed: Yes
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Former Professors > Chair Physical Chemistry II - Univ.-Prof. Dr. Andreas Fery
Faculties
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Physical Chemistry II
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Former Professors
Result of work at the UBT: Yes
DDC Subjects: 500 Science > 500 Natural sciences
500 Science > 530 Physics
500 Science > 540 Chemistry
Date Deposited: 08 Dec 2014 15:46
Last Modified: 08 Dec 2014 15:46
URI: https://eref.uni-bayreuth.de/id/eprint/3994