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Single-Molecule Spectroscopy on RC-LH1 Complexes of Rhodopseudomonas acidophila Strain 10050

Title data

Böhm, Paul S. ; Southall, June ; Cogdell, Richard J. ; Köhler, Jürgen:
Single-Molecule Spectroscopy on RC-LH1 Complexes of Rhodopseudomonas acidophila Strain 10050.
In: The Journal of Physical Chemistry B. Vol. 117 (2013) Issue 11 . - pp. 3120-3126.
ISSN 1520-5207
DOI: https://doi.org/10.1021/jp4005218

Abstract in another language

We have revisited the RC-LH1 complex from Rhodopseudomonas (Rps.) acidophila for single-molecule spectroscopy. For the current study the pigmentprotein complexes were stabilized in the detergent buffer solution using a relatively mild detergent (dodecyl-â-d-maltoside (DDM) instead of lauryldimethylamine N-oxide (LDAO)). This leads to a significant reduction of the fraction of broken/dissociated RC-LH1 complexes with respect to previous studies and has allowed us to investigate a sufficiently large sample of individual RC-LH1 complexes. For most of the complexes the fluorescenceexcitation spectra exhibit a narrow spectral feature at the red end of the spectrum. Analysis of the statistics of the spectral properties yields a close resemblance with the results obtained on RC-LH1 complexes from Rps. palustris for which a low-resolution X-ray structure is available. Based on this comparison we come to the conclusion that for both species the RC-LH1 complex can be described by the same structural model, that is, an overall elliptical assembly of pigments that features a gap.

Further data

Item Type: Article in a journal
Refereed: Yes
Institutions of the University: Faculties > Faculty of Mathematics, Physics und Computer Science > Department of Physics > Lehrstuhl Experimentalphysik IX - Spektroskopie weicher Materie > Lehrstuhl Experimentalphysik IX - Spektroskopie weicher Materie - Univ.-Prof. Dr. Jürgen Köhler
Faculties
Faculties > Faculty of Mathematics, Physics und Computer Science
Faculties > Faculty of Mathematics, Physics und Computer Science > Department of Physics
Faculties > Faculty of Mathematics, Physics und Computer Science > Department of Physics > Lehrstuhl Experimentalphysik IX - Spektroskopie weicher Materie
Result of work at the UBT: Yes
DDC Subjects: 500 Science > 530 Physics
Date Deposited: 11 Apr 2018 09:12
Last Modified: 11 Apr 2018 09:12
URI: https://eref.uni-bayreuth.de/id/eprint/43277