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AFM characterization of spin-coated multilayered dry lipid films prepared from aqueous vesicle suspensions

Title data

Krapf, Lisa ; Dezi, Manuela ; Reichstein, Werner ; Köhler, Jürgen ; Oellerich, Silke:
AFM characterization of spin-coated multilayered dry lipid films prepared from aqueous vesicle suspensions.
In: Colloids and Surfaces B : Biointerfaces. Vol. 82 (2011) Issue 1 . - pp. 25-32.
ISSN 0927-7765
DOI: https://doi.org/10.1016/j.colsurfb.2010.08.006

Abstract in another language

We present a detailed AFM study on multilayered dry lipid films prepared from aqueous vesicle suspensions. Different preparation techniques were applied in order to optimize the preparation of homogeneous lipid films of various film thicknesses. Suspensions of preformed DOPC/DPPC vesicles were adsorbed onto indium tin oxide-coated glass coverslips, a substrate also commonly employed for the formation of giant liposomes. We found that the homogeneity of the lipid films could substantially be improved when applying a spin-coating step during the film preparation. These films were much more homogeneous than those prepared by conventional drop-casting and in addition the film thickness could be controlled. When using a combination of vesicle adsorption and spin-coating the quality and thickness of the films depended crucially on the lipid concentration of the vesicle suspension, the adsorption temperature and the adsorption time. For lipid films prepared by direct spin-coating the lipid concentration and the applied spin-coating sequence were critical parameters for the quality and thickness of the deposited lipid films.

Further data

Item Type: Article in a journal
Refereed: Yes
Keywords: Spin-Coating
Institutions of the University: Faculties > Faculty of Mathematics, Physics und Computer Science > Department of Physics > Lehrstuhl Experimentalphysik IX - Spektroskopie weicher Materie > Lehrstuhl Experimentalphysik IX - Spektroskopie weicher Materie - Univ.-Prof. Dr. Jürgen Köhler
Faculties
Faculties > Faculty of Mathematics, Physics und Computer Science
Faculties > Faculty of Mathematics, Physics und Computer Science > Department of Physics
Faculties > Faculty of Mathematics, Physics und Computer Science > Department of Physics > Lehrstuhl Experimentalphysik IX - Spektroskopie weicher Materie
Result of work at the UBT: Yes
DDC Subjects: 500 Science > 530 Physics
Date Deposited: 11 Apr 2018 06:57
Last Modified: 11 Apr 2018 06:57
URI: https://eref.uni-bayreuth.de/id/eprint/43356