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Cooperative binding of ATP and RNA induces a closed conformation in a DEAD box RNA helicase

Title data

Theißen, Bettina ; Karow, Anne R. ; Köhler, Jürgen ; Gubaev, Airat ; Klostermeier, Dagmar:
Cooperative binding of ATP and RNA induces a closed conformation in a DEAD box RNA helicase.
In: Proceedings of the National Academy of Sciences of the United States of America. Vol. 105 (2008) Issue 2 . - pp. 548-553.
ISSN 1091-6490
DOI: https://doi.org/10.1073/pnas.0705488105

Abstract in another language

RNA helicases couple the energy from ATP hydrolysis with structural changes of their RNA substrates. DEAD box helicases form the largest class of RNA helicases and share a helicase core comprising two RecA-like domains. An opening and closing of the interdomain cleft during RNA unwinding has been postulated but not shown experimentally. Single-molecule FRET experiments with the Bacillus subtilis DEAD box helicase YxiN carrying donor and acceptor fluorophores on different sides of the interdomain cleft reveal an open helicase conformation in the absence of nucleotides, or in the presence of ATP, or ADP, or RNA. In the presence of ADP and RNA, the open conformation is retained. By contrast, cooperative binding of ATP and RNA leads to a compact helicase structure, proving that the ATP- and ADP-bound states of RNA helicases display substantially different structures only when the RNA substrate is bound. These results establish a closure of the interdomain cleft in the helicase core at the beginning of the unwinding reaction, and suggest a conserved mechanism of energy conversion among DEAD box helicases across kingdoms.

Further data

Item Type: Article in a journal
Refereed: Yes
Keywords: YxiN
Institutions of the University: Faculties > Faculty of Mathematics, Physics und Computer Science > Department of Physics > Lehrstuhl Experimentalphysik IX - Spektroskopie weicher Materie > Lehrstuhl Experimentalphysik IX - Spektroskopie weicher Materie - Univ.-Prof. Dr. Jürgen Köhler
Faculties
Faculties > Faculty of Mathematics, Physics und Computer Science
Faculties > Faculty of Mathematics, Physics und Computer Science > Department of Physics
Faculties > Faculty of Mathematics, Physics und Computer Science > Department of Physics > Lehrstuhl Experimentalphysik IX - Spektroskopie weicher Materie
Result of work at the UBT: Yes
DDC Subjects: 500 Science > 530 Physics
Date Deposited: 11 Apr 2018 12:38
Last Modified: 12 Oct 2018 06:41
URI: https://eref.uni-bayreuth.de/id/eprint/43406