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Apsite, Indra ; Uribe Gómez, Juan Manuel ; Posada Murcia, Andrés ; Rosenfeldt, Sabine ; Salehi, Sahar ; Ionov, Leonid:
4D biofabrication of skeletal muscle microtissues.
In: Biofabrication.
Bd. 12
(2020)
Heft 1
.
- 015016.
ISSN 1758-5090
DOI: https://doi.org/10.1088/1758-5090/ab4cc4
Abstract
Skeletal muscle is one of the most abundant tissues in the body. Although it has a relatively good regeneration capacity, it cannot heal in the case of disease or severe damage. Many current tissue engineering strategies fall short due to the complex structure of skeletal muscle. Biofabrication techniques have emerged as a popular set of methods for increasing the complexity of tissue-like constructs. In this paper, 4D biofabrication technique is introduced for fabrication of the skeletal muscle microtissues. To this end, a bilayer scaffold consisting of a layer of anisotropic methacrylated alginate fibers (AA-MA) and aligned polycaprolactone (PCL) fibers was fabricated using electrospinning and later induced to self-fold to encapsulate myoblasts. Bilayer mats undergo shape-transformation in aqueous buffer, a process that depends on their overall thickness, the thickness of each layer and the geometry of the mat. Proper selection of these parameters allowed fabrication of scroll-like tubes encapsulating myoblasts. The myoblasts were shown to align along the axis of the anisotropic PCL fibers and further differentiated into aligned myotubes that contracted under electrical stimulation. Overall the significance of this approach is in fabrication of hollow tubular constructs that can be further developed for formation of a vascularized and functional muscle.
Weitere Angaben
| Publikationsform: | Artikel in einer Zeitschrift |
|---|---|
| Begutachteter Beitrag: | Ja |
| Institutionen der Universität: | Fakultäten > Fakultät für Ingenieurwissenschaften > Professur Biofabrikation Fakultäten > Fakultät für Ingenieurwissenschaften > Professur Biofabrikation > Professur Biofabrikation - Univ.-Prof. Dr. Leonid Ionov Fakultäten Fakultäten > Fakultät für Ingenieurwissenschaften |
| Titel an der UBT entstanden: | Ja |
| Themengebiete aus DDC: | 500 Naturwissenschaften und Mathematik > 540 Chemie |
| Eingestellt am: | 16 Okt 2019 06:20 |
| Letzte Änderung: | 30 Mai 2023 11:34 |
| URI: | https://eref.uni-bayreuth.de/id/eprint/52771 |