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4D biofabrication of skeletal muscle microtissues

Title data

Apsite, Indra ; Uribe, Juan Manuel ; Posada, Andres Fernando ; Rosenfeldt, Sabine ; Salehi, Sahar ; Ionov, Leonid:
4D biofabrication of skeletal muscle microtissues.
In: Biofabrication. (2019) .
ISSN 1758-5090
DOI: https://doi.org/10.1088/1758-5090/ab4cc4

Official URL: Volltext

Abstract in another language

Skeletal muscle is one of the most abundant tissues in the body. Although it has a relatively good regeneration capacity, it cannot heal in the case of disease or severe damage. Many current tissue engineering strategies fall short due to the complex structure of skeletal muscle. Biofabrication techniques have emerged as a popular set of methods for increasing the complexity of tissue-like constructs. In this paper, 4D biofabrication technique is introduced for fabrication of the skeletal muscle microtissues. To this end, a bilayer scaffold consisting of a layer of anisotropic methacrylated alginate fibers (AA-MA) and aligned polycaprolactone (PCL) fibers was fabricated using electrospinning and later induced to self-fold to encapsulate myoblasts. Bilayer mats undergo shape-transformation in aqueous buffer, a process that depends on their overall thickness, the thickness of each layer and the geometry of the mat. Proper selection of these parameters allowed fabrication of scroll-like tubes encapsulating myoblasts. The myoblasts were shown to align along the axis of the anisotropic PCL fibers and further differentiated into aligned myotubes that contracted under electrical stimulation. Overall the significance of this approach is in fabrication of hollow tubular constructs that can be further developed for formation of a vascularized and functional muscle.

Further data

Item Type: Article in a journal
Refereed: Yes
Institutions of the University: Faculties > Faculty of Engineering Science > Professur Biofabrikation
Faculties > Faculty of Engineering Science > Professur Biofabrikation > Professur Biofabrikation - Univ.-Prof. Dr. Leonid Ionov
Result of work at the UBT: Yes
DDC Subjects: 500 Science > 540 Chemistry
Date Deposited: 16 Oct 2019 06:20
Last Modified: 16 Oct 2019 06:20
URI: https://eref.uni-bayreuth.de/id/eprint/52771