bei Google ScholarTitelangaben
Pauli, Andrea ; Althoff, Friederike ; Oliveira, Raquel A. ; Heidmann, Stefan ; Schuldiner, Oren ; Lehner, Christian F. ; Dickson, Barry J. ; Nasmyth, Kim:
Cell-type-specific TEV protease cleavage reveals cohesin functions in Drosophila neurons.
In: Developmental Cell.
Bd. 14
(2008)
Heft 2
.
- S. 239-251.
ISSN 1878-1551
DOI: https://doi.org/10.1016/j.devcel.2007.12.009
Abstract
Cohesin is a highly conserved multisubunit complex that holds sister chromatids together in mitotic cells. At the metaphase to anaphase transition, proteolytic cleavage of the alpha kleisin subunit (Rad21) by separase causes cohesin's dissociation from chromosomes and triggers sister-chromatid disjunction. To investigate cohesin's function in postmitotic cells, where it is widely expressed, we have created fruit flies whose Rad21 can be cleaved by TEV protease. Cleavage causes precocious separation of sister chromatids and massive chromosome missegregation in proliferating cells, but not disaggregation of polytene chromosomes in salivary glands. Crucially, cleavage in postmitotic neurons is lethal. In mushroom-body neurons, it causes defects in axon pruning, whereas in cholinergic neurons it causes highly abnormal larval locomotion. These data demonstrate essential roles for cohesin in nondividing cells and also introduce a powerful tool by which to investigate protein function in metazoa.
Weitere Angaben
| Publikationsform: | Artikel in einer Zeitschrift |
|---|---|
| Begutachteter Beitrag: | Ja |
| Keywords: | Sister-Chromatid Cohesion; De-Lange-Syndrome; Gene-Expression; Axonal Migrations; Turning Behavior; SMC Proteins; Nipped-B; CHromosome; Homolog; Localization |
| Institutionen der Universität: | Fakultäten Fakultäten > Fakultät für Biologie, Chemie und Geowissenschaften > Fachgruppe Biologie > Lehrstuhl Genetik Fakultäten > Fakultät für Biologie, Chemie und Geowissenschaften Fakultäten > Fakultät für Biologie, Chemie und Geowissenschaften > Fachgruppe Biologie |
| Titel an der UBT entstanden: | Nein |
| Themengebiete aus DDC: | 500 Naturwissenschaften und Mathematik > 570 Biowissenschaften; Biologie |
| Eingestellt am: | 05 Mär 2020 10:08 |
| Letzte Änderung: | 26 Okt 2022 12:35 |
| URI: | https://eref.uni-bayreuth.de/id/eprint/54470 |