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Condensin I binds chromatin early in prophase and displays a highly dynamic association with Drosophila mitotic chromosomes

Title data

Oliveira, Raquel A. ; Heidmann, Stefan ; Sunkel, Claudio E.:
Condensin I binds chromatin early in prophase and displays a highly dynamic association with Drosophila mitotic chromosomes.
In: Chromosoma. Vol. 116 (2007) Issue 3 . - pp. 259-274.
ISSN 1432-0886
DOI: https://doi.org/10.1007/s00412-007-0097-5

Abstract in another language

The condensed state of mitotic chromosomes is crucial for faithful genome segregation. Key factors implicated in the formation of mitotic chromosomes are the condensin I and II complexes. In Drosophila, condensin I appears to play a major role in mitotic chromosome organization. To analyze its dynamic behavior, we expressed Barren, a condensin I non-Structural Maintenance of Chromosomes subunit, as a fully functional enhanced green fluorescent protein (EGFP) fusion protein in the female and followed it during early embryonic divisions. We find that, in Drosophila, Barren-EGFP associates with chromatin early in prophase concomitantly with the initiation of chromosome condensation. Barren-EGFP loading starts at the centromeric region from where it spreads distally reaching maximum accumulation at metaphase/early anaphase. Fluorescence Recovery After Photobleaching analysis indicates that most of the bound protein exchanges rapidly with the cytoplasmic pool during prometaphase/metaphase. Taken together, our results suggest that in Drosophila, condensin I is involved in the initial stages of chromosome condensation. Furthermore, the rapid turnover of Barren-EGFP indicates that the mechanism by which condensin I promotes mitotic chromosome organization is inconsistent with a static scaffold model.

Further data

Item Type: Article in a journal
Refereed: Yes
Keywords: Aurora-B Kinase; Topoisomerase-II; Structural Integrity; Sister Chromatids; Gene-Expression; 13S Condensin; SMC Proteins; Subunit; Architecture; DNA
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Chair Genetics
Result of work at the UBT: No
DDC Subjects: 500 Science > 570 Life sciences, biology
Date Deposited: 05 Mar 2020 10:46
Last Modified: 05 Mar 2020 10:46
URI: https://eref.uni-bayreuth.de/id/eprint/54472