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Degradation of Drosophila PIM regulates sister chromatid separation during mitosis

Title data

Leismann, Oliver ; Herzig, Alf ; Heidmann, Stefan ; Lehner, Christian F.:
Degradation of Drosophila PIM regulates sister chromatid separation during mitosis.
In: Genes & Development. Vol. 14 (2000) Issue 17 . - pp. 2192-2205.
ISSN 1549-5477
DOI: https://doi.org/10.1101/gad.176700

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Abstract in another language

Drosophila Pimples (PIM) and Three rows (THR) are required for sister chromatid separation in mitosis. PIM accumulates during interphase and is degraded rapidly during mitosis. This degradation is dependent on a destruction box similar to that of B-type cyclins. Nondegradable PIM with a mutant destruction box call rescue sister chromatid separation in pim mutants but only when expressed at low levels. Higher levels of nondegradable PIM, as well as overexpression of wild-type PIM, inhibit sister chromatid separation. Moreover, cells arrested in mitosis before sister chromatid separation (by colcemid or by mutations in fizzy/CDC20) fail to degrade PIM. Thus, although not related by primary sequence, PIM has intriguing functional similarities to the securin proteins of budding yeast, fission yeast, and vertebrates. Whereas these securins are known to form a complex with separins, we show that PIM associates in vivo with THR, which does not contain the conserved separin domain.

Further data

Item Type: Article in a journal
Refereed: Yes
Keywords: Mitosis; sister chromatid separation; securin; separin; pimples; three rows
anaphase-promoting complex; checkpoint protein MAD2; transforming gene PTTG; cyclin-A; saccharomyces-cerevisiae; budding yeast; fission yeast; cell-cycle; spindle checkpoint; CUT2 proteolysis
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Chair Genetics
Result of work at the UBT: Yes
DDC Subjects: 500 Science > 570 Life sciences, biology
Date Deposited: 06 Mar 2020 11:23
Last Modified: 06 Mar 2020 11:23
URI: https://eref.uni-bayreuth.de/id/eprint/54538