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Kinetoplastid RNA editing does not require the terminal 3' hydroxyl of guide RNA, but modifications to the guide RNA terminus can inhibit in vitro U insertion

Title data

Burgess, Moffett L. K. ; Heidmann, Stefan ; Stuart, Kenneth:
Kinetoplastid RNA editing does not require the terminal 3' hydroxyl of guide RNA, but modifications to the guide RNA terminus can inhibit in vitro U insertion.
In: RNA : a Publication of the RNA Society. Vol. 5 (July 1999) Issue 7 . - pp. 883-892.
ISSN 1469-9001
DOI: https://doi.org/10.1017/s1355838299990453

Abstract in another language

During RNA editing in kinetoplastid parasites, trans-acting guide RNAs (gRNAs) direct the insertion and deletion of U residues at precise sites in mitochondrial pre-mRNAs, We show here that some modifications to the 3' terminal ribose of gRNA inhibit its ability to direct in vitro U insertion. However, we found that gRNAs lacking this moiety in some circumstances support in vitro editing. Thus, the 3' OH is not required. Inhibition resulting from gRNA modification can be overcome by increasing the gRNA-pre-mRNA base-pairing potential upstream of the editing site, suggesting an importance for this interaction to productive processing.

Further data

Item Type: Article in a journal
Refereed: No
Keywords: guide RNA; Kinetoplastidae; RNA editing; trypanosomes; Trypanosoma-Brucei; Messenger-RNA; In-Vitro; Mitochondria; Mechanism; Molecules; Cleavage; Proteins; Information; Nucleotides
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Chair Genetics
Result of work at the UBT: No
DDC Subjects: 500 Science > 570 Life sciences, biology
Date Deposited: 10 Mar 2020 12:26
Last Modified: 10 Mar 2020 12:26
URI: https://eref.uni-bayreuth.de/id/eprint/54551