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Burgess, Moffett L. K. ; Heidmann, Stefan ; Stuart, Kenneth:
Kinetoplastid RNA editing does not require the terminal 3' hydroxyl of guide RNA, but modifications to the guide RNA terminus can inhibit in vitro U insertion.
In: RNA : a Publication of the RNA Society.
Bd. 5
(1999)
Heft 7
.
- S. 883-892.
ISSN 1469-9001
DOI: https://doi.org/10.1017/s1355838299990453
Abstract
During RNA editing in kinetoplastid parasites, trans-acting guide RNAs (gRNAs) direct the insertion and deletion of U residues at precise sites in mitochondrial pre-mRNAs, We show here that some modifications to the 3' terminal ribose of gRNA inhibit its ability to direct in vitro U insertion. However, we found that gRNAs lacking this moiety in some circumstances support in vitro editing. Thus, the 3' OH is not required. Inhibition resulting from gRNA modification can be overcome by increasing the gRNA-pre-mRNA base-pairing potential upstream of the editing site, suggesting an importance for this interaction to productive processing.
Weitere Angaben
| Publikationsform: | Artikel in einer Zeitschrift |
|---|---|
| Begutachteter Beitrag: | Nein |
| Keywords: | guide RNA; Kinetoplastidae; RNA editing; trypanosomes; Trypanosoma-Brucei; Messenger-RNA; In-Vitro; Mitochondria; Mechanism; Molecules; Cleavage; Proteins; Information; Nucleotides |
| Institutionen der Universität: | Fakultäten > Fakultät für Biologie, Chemie und Geowissenschaften > Fachgruppe Biologie Fakultäten > Fakultät für Biologie, Chemie und Geowissenschaften > Fachgruppe Biologie > Lehrstuhl Genetik Fakultäten Fakultäten > Fakultät für Biologie, Chemie und Geowissenschaften |
| Titel an der UBT entstanden: | Nein |
| Themengebiete aus DDC: | 500 Naturwissenschaften und Mathematik > 570 Biowissenschaften; Biologie |
| Eingestellt am: | 10 Mär 2020 12:26 |
| Letzte Änderung: | 26 Okt 2022 12:32 |
| URI: | https://eref.uni-bayreuth.de/id/eprint/54551 |