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Association of guide RNA binding protein gBP21 with active RNA editing complexes in Trypanosoma brucei

Title data

Allen, Thomas E. ; Heidmann, Stefan ; Reed, RoseMary ; Myler, Peter J. ; Göringer, H. Ulrich ; Stuart, Kenneth D.:
Association of guide RNA binding protein gBP21 with active RNA editing complexes in Trypanosoma brucei.
In: Molecular and Cellular Biology. Vol. 18 (October 1998) Issue 10 . - pp. 6014-6022.
ISSN 1098-5549
DOI: https://doi.org/10.1128/mcb.18.10.6014

Abstract in another language

RNA editing in Trypanosoma brucei mitochondria produces mature mRNAs by a series of enzyme-catalyzed reactions that specifically insert or delete uridylates in association with a macromolecular complex. Using a mitachondrial fraction enriched for in vitro RNA editing activity, we produced several monoclonal antibodies that are specific for a 21-kDa guide RNA (gRNA) binding protein initially identified by UV cross-linking. Immunofluorescence studies localize the protein to the mitochondrion, with a preference for the kinetoplast. The antibodies cause a supershift of preciously identified gRNA-specific ribonucleoprotein complexes and immunoprecipitate in vitro RNA editing activities that insert and delete uridylates. The immunoprecipitated material also contains gRNA-specific endoribonuclease, terminal uridylyltransferase, and RNA ligase activities as well as gRNA and both edited and unedited mRNA. The immunoprecipitate contains numerous proteins, of which the 21-kDa protein, a 90-kDa protein, and novel 55- and 16-kDa proteins can be UV cross-linked to gRNA, These studies indicate that the 21-kDa protein associates with the ribonucleoprotein complex (or complexes) that catalyze RNA editing.

Further data

Item Type: Article in a journal
Refereed: Yes
Keywords: Mitochondrial Ribonucleoprotein Complexes; Precursor Messenger-RNA; Kinetoplastid Mitochondria; In-Vitro; Molecules; Grna; Information; Transcript; Mechanism; Model
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Chair Genetics
Result of work at the UBT: No
DDC Subjects: 500 Science > 570 Life sciences, biology
Date Deposited: 10 Mar 2020 12:36
Last Modified: 10 Mar 2020 12:36
URI: https://eref.uni-bayreuth.de/id/eprint/54552