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Wang, Bingbing ; Salavati, Reza ; Heidmann, Stefan ; Stuart, Kenneth:
A hammerhead ribozyme substrate and reporter for in vitro kinetoplastid RNA editing.
In: RNA : a Publication of the RNA Society.
Bd. 8
(2002)
Heft 4
.
- S. 548-554.
ISSN 1469-9001
DOI: https://doi.org/10.1017/s135583820202962x
Abstract
Current in vitro assays for RNA editing in kinetoplastids directly examine the products generated by Incubation of pre-mRNA substrate with guide RNA (gRNA) and mitochondrial (mt) extract. RNA editing substrates that are modeled on hammerhead ribozymes were designed with catalytic cores that contained or lacked additional uridylates (Us). They proved to be sensitive reporters of editing activity when used for in vitro assays. A deletion editing substrate that is based on A6 pre-mRNA had no ribozyme activity, but its incubation with gRNA and mt extract resulted In its deletion editing and production of a catalytically active ribozyme. Hammerhead ribozymes are thus sensitive tools to assay in vitro RNA editing.
Weitere Angaben
| Publikationsform: | Artikel in einer Zeitschrift |
|---|---|
| Begutachteter Beitrag: | Ja |
| Keywords: | gRNA; Trypanosoma brucei; uridylate deletion; uridylate insertion; Precursor Messenger-RNA; Trypaosoma-Brucei; Guide RNAS; In-Vitro; Cleavage; GRNA; Mitochondria; Mechanism; Specificity; Efficiency |
| Institutionen der Universität: | Fakultäten > Fakultät für Biologie, Chemie und Geowissenschaften > Fachgruppe Biologie Fakultäten > Fakultät für Biologie, Chemie und Geowissenschaften > Fachgruppe Biologie > Professur Genetik Fakultäten Fakultäten > Fakultät für Biologie, Chemie und Geowissenschaften |
| Titel an der UBT entstanden: | Nein |
| Themengebiete aus DDC: | 500 Naturwissenschaften und Mathematik > 570 Biowissenschaften; Biologie |
| Eingestellt am: | 10 Mär 2020 13:07 |
| Letzte Änderung: | 26 Okt 2022 12:33 |
| URI: | https://eref.uni-bayreuth.de/id/eprint/54598 |