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RNA editing : A mechanism for gRNA-specified uridylate insertion into precursor mRNA

Title data

Kable, Moffett L. ; Seiwert, Scott D. ; Heidmann, Stefan ; Stuart, Kenneth:
RNA editing : A mechanism for gRNA-specified uridylate insertion into precursor mRNA.
In: Science. Vol. 273 (30 August 1996) Issue 5279 . - pp. 1189-1195.
ISSN 1095-9203
DOI: https://doi.org/10.1126/science.273.5279.1189

Abstract in another language

In the mitochondria of trypanosomatid protozoa the precursors of messenger RNAs (pre-mRNAs) have their coding information remodeled by the site-specific insertion and deletion of uridylate (U) residues. Small trans-acting guide RNAs (gRNAs) supply the genetic information for this RNA editing. An in vitro system was developed to study the mechanism of U insertion into pre-mRNA. U-insertion editing occurs through a series of enzymatic steps that begin with gRNA-directed pre-mRNA cleavage. Inserted U's are derived from free uridine triphosphate and are added to the 3' terminus of a 5' pre-mRNA cleavage product. gRNA specifies edited RNA sequence at the subsequent ligation step by base pairing-mediated juxtaposition of the 3' cleavage product and the processed 5' cleavage product. gRNA/pre-mRNA chimeras, purported intermediates, seem to be abortive end products of the same reaction.

Further data

Item Type: Article in a journal
Refereed: Yes
Keywords: Messenger-RNA; Trypanosoma-Brucei; Leishmania-Tarentolae; Crithidia-Fasciculata; Guide RNAS; Kinetoplastid Mitochondria; Chimera Formation; In-Vitro; Domains; Ligase
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Chair Genetics
Result of work at the UBT: No
DDC Subjects: 500 Science > 570 Life sciences, biology
Date Deposited: 12 Mar 2020 12:58
Last Modified: 12 Mar 2020 12:58
URI: https://eref.uni-bayreuth.de/id/eprint/54609