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Direct visualization of uridylate deletion in vitro suggests a mechanism for kinetoplastid RNA editing

Title data

Seiwert, Scott D. ; Heidmann, Stefan ; Stuart, Kenneth:
Direct visualization of uridylate deletion in vitro suggests a mechanism for kinetoplastid RNA editing.
In: Cell. Vol. 84 (22 March 1996) Issue 6 . - pp. 831-841.
ISSN 0092-8674
DOI: https://doi.org/10.1016/S0092-8674(00)81062-4

Abstract in another language

Deletion of uridylates from the 3'-most editing site of synthetic ATPase 6 pre-mRNA can be visualized directly by coincubation of a radiolabeled substrate RNA and a synthetic gRNA in 20S fractions of T. brucei mitochondrial lysates. Substrate RNA cleavage is gRNA directed and occurs 3' to the uridylates to be deleted. U residues appear to be sequentially removed from the 3' end of the 5' cleavage product prior to religation of the two pre-mRNA halves. gRNA/mRNA chimeric molecules are also produced. Time course experiments indicate that chimeras appear after cleavage intermediates and edited product. Furthermore, a mutant gRNA promotes formation of edited product but not detectable chimeras. Our results suggest a model for kinetoplastid RNA editing in which chimeric molecules are nonproductive end products of editing and not intermediates that serve as a repository for deleted U's.

Further data

Item Type: Article in a journal
Refereed: No
Keywords: Mitochondrial Extracts; Leishmania-Tarentolae; Messenger-RNAS; Molecules; Invitro; Sites
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Chair Genetics
Result of work at the UBT: No
DDC Subjects: 500 Science > 570 Life sciences, biology
Date Deposited: 12 Mar 2020 13:04
Last Modified: 12 Mar 2020 13:04
URI: https://eref.uni-bayreuth.de/id/eprint/54610