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Seiwert, Scott D. ; Heidmann, Stefan ; Stuart, Kenneth:
Direct visualization of uridylate deletion in vitro suggests a mechanism for kinetoplastid RNA editing.
In: Cell.
Bd. 84
(1996)
Heft 6
.
- S. 831-841.
ISSN 1097-4172
DOI: https://doi.org/10.1016/S0092-8674(00)81062-4
Abstract
Deletion of uridylates from the 3'-most editing site of synthetic ATPase 6 pre-mRNA can be visualized directly by coincubation of a radiolabeled substrate RNA and a synthetic gRNA in 20S fractions of T. brucei mitochondrial lysates. Substrate RNA cleavage is gRNA directed and occurs 3' to the uridylates to be deleted. U residues appear to be sequentially removed from the 3' end of the 5' cleavage product prior to religation of the two pre-mRNA halves. gRNA/mRNA chimeric molecules are also produced. Time course experiments indicate that chimeras appear after cleavage intermediates and edited product. Furthermore, a mutant gRNA promotes formation of edited product but not detectable chimeras. Our results suggest a model for kinetoplastid RNA editing in which chimeric molecules are nonproductive end products of editing and not intermediates that serve as a repository for deleted U's.
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| Publikationsform: | Artikel in einer Zeitschrift |
|---|---|
| Begutachteter Beitrag: | Nein |
| Keywords: | Mitochondrial Extracts; Leishmania-Tarentolae; Messenger-RNAS; Molecules; Invitro; Sites |
| Institutionen der Universität: | Fakultäten > Fakultät für Biologie, Chemie und Geowissenschaften > Fachgruppe Biologie > Lehrstuhl Genetik Fakultäten Fakultäten > Fakultät für Biologie, Chemie und Geowissenschaften Fakultäten > Fakultät für Biologie, Chemie und Geowissenschaften > Fachgruppe Biologie |
| Titel an der UBT entstanden: | Nein |
| Themengebiete aus DDC: | 500 Naturwissenschaften und Mathematik > 570 Biowissenschaften; Biologie |
| Eingestellt am: | 12 Mär 2020 13:04 |
| Letzte Änderung: | 06 Sep 2023 11:44 |
| URI: | https://eref.uni-bayreuth.de/id/eprint/54610 |