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Flexibility and interchangeability of polyadenylation signals in Saccharomyces cerevisiae

Title data

Heidmann, Stefan ; Schindewolf, C. ; Stumpf, G. ; Domdey, H.:
Flexibility and interchangeability of polyadenylation signals in Saccharomyces cerevisiae.
In: Molecular and Cellular Biology. Vol. 14 (July 1994) Issue 7 . - pp. 4633-4642.
ISSN 1098-5549
DOI: https://doi.org/10.1128/MCB.14.7.4633

Abstract in another language

Various signal motifs have been reported to be essential for proper mRNA 3'-end formation in the yeast Saccharomyces cerevisiae. However, none of these motifs has been shown to be sufficient to direct 3'-end processing and/or transcription termination. Therefore, several structural motifs have to act in concert for efficient 3'-end formation. In the region upstream of the three polyadenylation sites of the yeast gene for alcohol dehydrogenase I (ADH1), we have identified a hitherto unknown signal sequence contained within the octamer AAAAAAAA. This motif, located 11 nucleotides upstream of the first ADH1 polyadenylation site, is responsible for the utilization of this site in vitro and in vivo, since mutational alteration drastically reduced 3'-end formation at this position. Insertion of 38 ADH1-derived nucleotides encompassing the (A), motif into the 3'-end formation deficient cyc1-512 deletion mutant restored full processing capacity in vitro. Insertion of the octamer alone did not restore 3'-end formation, although mutation of the (A), motif in the functional construct had abolished 3'-end processing activity almost completely. This demonstrates that the sequence AAAAAAAA is a necessary, although not sufficient, signal for efficient mRNA 3'-end formation in S. cerevisiae.

Further data

Item Type: Article in a journal
Refereed: Yes
Keywords: CYC1 Messenger-RNA; 3' END; Escherichia-Coli; Transcription Termination; Sequence Elements; 3'-END Formation; Poly(A) Site; Yeast; Gene; Invitro
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Chair Genetics
Result of work at the UBT: No
DDC Subjects: 500 Science > 570 Life sciences, biology
Date Deposited: 12 Mar 2020 13:17
Last Modified: 12 Mar 2020 13:17
URI: https://eref.uni-bayreuth.de/id/eprint/54611