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Identification of pre-mRNA polyadenylation sites in Saccharomyces cerevisiae

Title data

Heidmann, Stefan ; Obermaier, B. ; Vogel, K. ; Domdey, H.:
Identification of pre-mRNA polyadenylation sites in Saccharomyces cerevisiae.
In: Molecular and Cellular Biology. Vol. 12 (September 1992) Issue 9 . - pp. 4215-4299.
ISSN 1098-5549
DOI: https://doi.org/10.1128/mcb.12.9.4215

Abstract in another language

In contrast to higher eukaryotes, little is known about the nature of the sequences which direct 3'-end formation of pre-mRNAs in the yeast Saccharomyces cerevisiae. The hexanucleotide AAUAAA, which is highly conserved and crucial in mammals, does not seem to have any functional importance for 3'-end formation in yeast cells. Instead, other elements have been proposed to serve as signal sequences. We performed a detailed investigation of the yeast ACT1, ADH1, CYC1, and YPT1 cDNAs, which showed that the polyadenylation sites used in vivo can be scattered over a region spanning up to 200 nucleotides. It therefore seems very unlikely that a single signal sequence is responsible for the selection of all these polyadenylation sites. Our study also showed that in the large majority of mRNAs, polyadenylation starts directly before or after an adenosine residue and that 3'-end formation of ADH1 transcripts occurs preferentially at the sequence PyAAA. Site-directed mutagenesis of these sites in the ADH1 gene suggested that this PyAAA sequence is essential for polyadenylation site selection both in vitro and in vivo. Furthermore, the 3'-terminal regions of the yeast genes investigated here are characterized by their capacity to act as signals for 3'-end formation in vivo in either orientation.

Further data

Item Type: Article in a journal
Refereed: Yes
Keywords: 3' END Formation; Escherichia-Coli; Transcription Termination; Schizosaccharomyces-Pombe; Point Mutations; Poly(A) site; Actin Gene; Yeast; Invitro; DNA
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Chair Genetics
Result of work at the UBT: No
DDC Subjects: 500 Science > 570 Life sciences, biology
Date Deposited: 12 Mar 2020 13:22
Last Modified: 12 Mar 2020 13:22
URI: https://eref.uni-bayreuth.de/id/eprint/54612